Phytochemical and Pharmacological Evaluation along with Antimicrobial Properties of Gynura Procumbens Leaves Extract

Abstract

The accentuated growth inside the use of Gynura procumbens go away as suitable for eating leave and herbal medicinal drug to deal with sicknesses makes its miles essential to evaluate facts available on its antibacterial, antifungal, phytochemical screening and antioxidant activities. G. procumbens leaves methanol and ethyl acetate crude extract was prepared via the cold extraction approach. The methanolic G. procumbens extract became used to phytochemical assessments for plant secondary metabolites; carbohydrates, alkaloids, phenolic compounds, flavonoids, tannins, glycosides, steroids, proteins, acidic compounds and saponins using preferred processes techniques. Antibacterial activities of G. procumbens extracts was investigated by using agar well diffusion method and antifungal activities by using the disc-diffusion method. G. procumbens extracts antioxidant activity was assessed via DPPH assay to measure its free radical scavenging capability. Phytochemical screening revealed that presence of carbohydrate, alkaloids, flavonoids, glycosides, phenolic compounds and tannins but saponins, proteins, acidic compounds and steroids were not found. Antimicrobial investigation showed that only ethyl acetate extracts have the mild sensitivity to almost all the bacteria and fungi (except Escherichia coli), whereas methanol extracts did not demonstrate any antimicrobial property. The average zones of inhibition produced by ethyl acetate extracts were found to be 6-7 mm at a concentration of 400μg/disc. Antioxidant study showed that IC50 of ethyl acetate extract of G. procumbens leaves was 322.244 μg/ml whereas the IC50 of standard ascorbic acid was 41.683 μg/ml. From the present experiment it could be concluded that G. Procumbens is the source of alkaloids, flavonoids, tannins and it’s has also antimicrobial, antioxidant properties. These findings imply that G. Procumbens leaves extracts is a good medicinal supply or herbal properties that may have high therapeutic value.