Physicochemical Analysis of Ginger (Zingiber officinale Rosc.) Rhizome along with its TLC, HPLC and HPTLC Profile

Abstract

Introduction: Ginger (Zingiber officinale Rosc.) is a well-known traditional medicinal plant used for therapeutic effects in both Unani and Ayurvedic System of Medicine. It has been founded to have many therapeutic activities such as anti-inflammatory, anti-rheumatic and anti-gout properties. It is also useful in the management of cough, dyspepsia, loss of appetite, relaxed sore-throat, retching, spasms / spasmodic affections of the stomach, vomiting. The present study aimed towards the evaluation of the parameters involved in the determination of the quality and purity of Zingiber officinale Rosc. rhizome and its standardization. Methods: Organoleptic characters, extractive values, ash values, phyto-chemical analysis, TLC, HPTLC, fluorescence analysis and HPLC profile etc. were the parameters used for the standardisation of the test drug. Results: Total ash values, water, alcohol and Ether soluble extractive values and volatile oil percentage was found to be 7.60%, 11.23%, 8.55%, 2.5% and 3.75% respectively. TLC profile of Z. officinale Rosc shows 05 and 13 spots in UV short and exposure in anisaldehyde-sulphuric acid respectively. The HPLC pattern shows 29 peaks and the peak no. 2, 7 and 5 are major peaks having area concentration and retention time as 9.64% at 2.66 min, 10.12% at 3.25 min. and 15.22% at 3.61 min. respectively. Conclusion: The study will provide referential information for the good quality, purity and identification for the future batches of Zingiber officinale Rosc.