POTENTIAL AMELIORATIVE ROLE OF N-ACETYLCYSTEINE AGAINST TESTICULAR DYSFUNCTION INDUCED BY TITANIUM DIOXIDE IN MALE ALBINO RATS

Abstract

In this study, we examined the ameliorative action of N-Acetylcysteine (NAC) against Titanium Dioxide (TiO2) induced testicular degeneration in albino ra ts. Adult male albino rats were given saline as a control group, TiO2 (1200 mg kg -1 BW), NAC (100 mg kg -1 BW) and co-treatment of NAC and TiO2 as a protective group for 3 months. Testicular tissues were extracted for changes in testicular gene expression and histopathology. Administration of Ti O2 significantly increased mRNA expression of IL-6 and TNF-α that are normalized by NAC administration. TiO2 ad ministration down regulated Glutathione-S-Transferase (GST) while increased B-cell Lymphoma2 (BcL2) expressions. Coadministration of rats by NAC together with TiO2 no rmalized changes in GST and BcL2 expression. Expression of steroidogenesis related genes [Androg en Binding Protein (ABR), 17 β-Hydroxysteroid Dehydrogenase (17 β-HSD), cytochrome P450 17A (CYP 17α ) and aromatase] showed down regulation in TiO2 administered groups and normalized when NAC given together with TiO2. Moreover, TiO2 induced toxicity in testes that accompanied by dege neration in seminiferous tubules with congestion, oedema and cell disruption that are partially norma lized by co-administration of NAC with TiO2. In conclusion, the present findings confirmed the benf icial effect of NAC to prevent apoptosis in spermatogenic and sertoli cells and testicular dysf unction induced by TiO2 in male albino rats.