Metabolism of [15N]Alanine in the Ectomycorrhizal Fungus Paxillus involutus

Michel Chalot, Roger D. Finlay, Hans Ek, Bengt Söderström
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引用次数: 10

Abstract

Chalot, M., Finlay, R. D., Ek, H., and Söderström, B. 1995. Metabolism of [15N]alanine in the ectomycorrhizal fungus Paxillus involutus. Experimental Mycology 19, 297-304. Alanine metabolism in the ectomycorrhizal fungus Paxillus involutus was investigated using [15N]alanine. Short-term exposure of mycelial discs to [15N]alanine showed that the greatest flow of 15N was to glutamate and to aspartate. Levels of enrichment were as high as 15-20% for glutamate and 13-18% for aspartate, whereas that of alanine reached 30%. Label was also detected in the amino-N of glutamine and in serine and glycine, although at lower levels. Preincubation of mycelia with aminooxyacetate, an inhibitor of transamination reactions. resulted in complete inhibition of the flow of the label to glutamate, aspartate, and amino-N of glutamine, whereas [15N]alanine rapidly accumulated. This evidence indicates the direct involvement of alanine aminotransferase for translocation of 15N from alanine to glutamate. Alanine may be a convenient reservoir of both nitrogen and carbon.

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[15N]丙氨酸在外生菌根真菌中代谢的研究
Chalot, M., Finlay, R. D., Ek, H.和Söderström, B. 1995。[15N]丙氨酸在外生菌根真菌中的代谢。真菌学通报,19(2):444 - 444。用[15N]丙氨酸研究了外生菌根真菌珙桐(Paxillus involutus)的丙氨酸代谢。短期暴露于[15N]丙氨酸的菌丝盘显示,15N的最大流量是谷氨酸和天冬氨酸。谷氨酸和天冬氨酸的富集水平分别高达15-20%和13-18%,而丙氨酸的富集水平达到30%。在谷氨酰胺的氨基氮、丝氨酸和甘氨酸中也检测到标签,尽管含量较低。用氨基乙酸预孵育菌丝,这是一种转氨化反应的抑制剂。导致标签向谷氨酰胺的谷氨酸、天冬氨酸和氨基氮的流动被完全抑制,而[15N]丙氨酸则迅速积累。这一证据表明丙氨酸转氨酶直接参与了15N从丙氨酸到谷氨酸的易位。丙氨酸可能是氮和碳的方便储存库。
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