Isolation and in vitro studies on indigenous fungi against root-knot nematode, Meloidogyne incognita

Q4 Agricultural and Biological Sciences Journal of Biological Control Pub Date : 2022-06-05 DOI:10.18311/jbc/2021/28554
Nithya Dharshini K., J. G, S. N., L. Rajendran
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Abstract

The present study was carried out to evaluate the indigenous fungi from soil against the root knot nematode, Meloidogyne incognita. Eleven isolates were isolated and screened for their efficacy against nematode under in vitro condition for egg hatching and juvenile mortality. The results revealed that the isolate F6 showed the highest egg hatching inhibition (88.5%) followed by F11 and F7 compared to control after incubation for 72 h. The highest juvenile mortality of 73% was recorded in isolate F6 followed by F11 and F9 after 72 h. Isolate F6 was morphologically and molecularly identified as Talaromyces pinophilus and F9 and F11 were identified as Aspergillus corrugates and Aspergillus nidulans. Among the fungi, T. pinophilus was found to be highly effective against root knot nematode.
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根结线虫(Meloidogyne incognita)本土真菌的分离与体外研究
研究了土壤中土生真菌对根结线虫(Meloidogyne incognita)的拮抗作用。在体外条件下对11株分离株进行了卵孵化和幼虫死亡率的筛选。结果表明,菌株F6孵育72 h后的卵孵化抑制率最高(88.5%),其次为F11和F7;菌株F6孵育72 h后的幼虫死亡率最高(73%),其次为F11和F9。菌株F6经形态学和分子鉴定为嗜松Talaromyces pinophilus,菌株F9和F11经鉴定为波曲霉(Aspergillus波纹曲霉)和假曲霉(Aspergillus nidulans)。其中,嗜pinophilus对根结线虫的防治效果较好。
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来源期刊
Journal of Biological Control
Journal of Biological Control Agricultural and Biological Sciences-Insect Science
CiteScore
0.50
自引率
0.00%
发文量
10
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