Study of the uroantiseptic activity of the complex of glycosides of phenolic compounds from lingonberry leaves in complex with the arginine amino acid

Abstract

The aim of the work – study the uroantiseptic activity of a complex of glycosides of phenolic compounds from lingonberry leaves in a complex with the amino acid arginine. Materials and methods. The object of the study was a phytosubstance, which is a complex of glycosides of phenolic compounds from lingonberry leaves in combination with the amino acid arginine (CGPA). Female laboratory rats were given a cleansing enema twice, after which the rats were provided with adequate sedation by injecting IV XYLA 2 % for anesthesia, then rectally injected 1000 μl of Escherichia coli strain at a concentration of 109 CFU/ml. The next day after infection, the animals were subjected to cryogenic action - acute cold stress at a temperature of 0+2 °C for 2.5 hours, promoting the development of the infectious and inflammatory process. The uroantiseptic effect was studied according to the following parameters: the number of leukocytes in the urine, the number of colony-forming microorganisms E.coli in 1 ml of urine (CFU/ml). Results. According to the obtained results of the experiment, it was established that the complex of glycosides of phenolic compounds from the leaves of lingonberry in a complex with the amino acid arginine (CGPA) showed a high uroantiseptic effect in the experimental treatment of urinary tract infection caused by E. coli ATCC 25922, which was manifested in the sanitation of the urinary tract from the uropathogen E. Coli. Conclusions. Analyzing the results, it can be concluded that on the model of urinary tract infection against the background of cryogenic exposure, CGPA at a dose of 100 mg/kg showed a uroantiseptic effect, which was stronger than the comparison drug "Inurek" and contributed to a faster disappearance of the titer of colony-forming microorganisms in 1 ml of urine about 2 days earlier than in the group that received dietary supplements of herbal origin "Inurek" and the disappearance of leukocyturia 3 days earlier compared to the group that received therapy with the comparison drug "Inurek". The obtained results indicate the prospects of further research of CGPA with the aim of creating new effective uroantiseptic agents of plant origin based on it