Simultaneous Determination and Mutual Interaction Study of Ciprofloxacin and Chloramphenicol in Concomitant Administration by a New UPLC Method

Abstract

A new simple, fast, accurate, precise and reproducible UPLC method was developed for the simultaneous estimation of ciprofloxacin and chloramphenicol. Reversed-phase Shim-pack XR-ODS (100 × 3.0 mm, 1.7 μm) column was used to resolve the drugs utilizing a mixture of CH3OH and 5 mM NaH2PO4 as mobile phase at gradient program equilibrated initially by 56:44 (v/v). The mobile phase was pumped at a flow rate of 0.20 mL min-1 with detection at 280 nm. 10 μL volume of sample was injected by the auto sampler. Separation was completed within 3.82 ± 0.03 minutes. For both drugs linear was obtained over a studied concentration range of 10 μg mL-1 with correlation coefficient 0.999. Relative Standard Deviation (RSD) for intra and inter day precision was <1.5% indicating the method’s good reproducibility. The mean recovery of the drug determination was 99.39%. LOD was found to be 0.025, 0.020 μg mL-1 for ciprofloxacin and chloramphenicol, respectively. The proposed method might be applied for routine analysis of both drugs in bulk and pharmaceutical formulations. Furthermore, no experimental evidences in favour of adverse pharmaceutical interaction were revealed in their concomitant use.