{"title":"Raman Spectroscopy of Bacterial Species and Strains Cultivated under Reproducible Conditions","authors":"J. Almarashi, N. Kapel, T. S. Wilkinson, H. Telle","doi":"10.1155/2012/540490","DOIUrl":null,"url":null,"abstract":"Rapid and reproducible discrimination between bacterial pathogens is a clear goal in microbiological laboratories when processing infected clinical samples. In this study Raman spectra were taken from at least 30 colonies of four strains of bacteria including Staphylococcus epidermidis (1457 and 9142) and Escherichia coli (K12 and Top 10) using the Renishaw in Via Raman microscope system. Analysis based on principal components suggests that even strain differentiation (e.g., 1457 versus 9142 or K12 versus Top10) is possible.","PeriodicalId":51163,"journal":{"name":"Spectroscopy-An International Journal","volume":"33 1","pages":"361-365"},"PeriodicalIF":0.0000,"publicationDate":"2012-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"35","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Spectroscopy-An International Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1155/2012/540490","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 35
Abstract
Rapid and reproducible discrimination between bacterial pathogens is a clear goal in microbiological laboratories when processing infected clinical samples. In this study Raman spectra were taken from at least 30 colonies of four strains of bacteria including Staphylococcus epidermidis (1457 and 9142) and Escherichia coli (K12 and Top 10) using the Renishaw in Via Raman microscope system. Analysis based on principal components suggests that even strain differentiation (e.g., 1457 versus 9142 or K12 versus Top10) is possible.
在处理感染的临床样本时，微生物实验室的一个明确目标是快速和可重复地区分细菌病原体。本研究利用Renishaw In Via拉曼显微镜系统采集了4株表皮葡萄球菌(1457和9142)和大肠杆菌(K12和Top 10)至少30个菌落的拉曼光谱。基于主成分的分析表明，即使应变分化(例如，1457与9142或K12与Top10)也是可能的。
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