Fe Element Analysis in the Spleen Tissue by Using EDS and ICP-MS after Aminated Silica Shelled Magnetite (Fe₃O₄) Administered to the Tail Vein of Mice

IF 0.6 4区材料科学 Q4 MATERIALS SCIENCE, MULTIDISCIPLINARY Journal of Magnetics Pub Date : 2022-12-31 DOI:10.4283/jmag.2022.27.4.388

M. Hasan, Jong-Gu Choi, Hafeza Akter, Sang-Suk Lee

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Abstract

The suppression of cytokine storm in severe coronavirus disease 2019 (COVID-19) patients can be treated with monoclonal antibody therapy against CD3 for T cell receptor inhibition. An optimized liquid phase as a CD3 antibody-magnetic nanoparticle (Ab-MNP) conjugate can inhibit the overactivation of T cells. We aim to ana-lyze the distribution of Fe in the spleen after acute administration of silica-conjugated amine magnetite (Fe3O4) nanoparticles (35 nm) delivered by intravenous injection. The Fe element distribution and concentration levels in spleen tissue were analyzed using energy dispersive spectroscopy (EDS) and inductively coupled plasma-mass spectrometry (ICP-MS). The experimental result is a difference in the concentration of Fe elements, which was 1.89×103 mg/kg in the spleen of a control mouse not administered with MNPs, whereas increases sig-nificantly to 1.93×103 mg/kg in that of a mouse administered with MNPs. Further, time kinetic analysis of bio-chemical and immunological parameters is required to confirm its suitability in bio-administration. © 2022 Journal of Magnetics.

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胺化二氧化硅壳磁铁矿(Fe₃O₄)给药小鼠尾静脉后脾组织铁元素的EDS和ICP-MS分析

针对T细胞受体抑制CD3单克隆抗体治疗可抑制COVID-19患者的细胞因子风暴。作为CD3抗体-磁性纳米颗粒(Ab-MNP)偶联物的优化液相可以抑制T细胞的过度活化。我们的目的是分析急性静脉注射二氧化硅共轭胺磁铁矿(Fe3O4)纳米颗粒(35 nm)后脾脏中铁的分布。采用能量色散光谱(EDS)和电感耦合等离子体质谱(ICP-MS)分析脾脏组织中铁元素的分布和浓度水平。实验结果显示，未给予MNPs的对照小鼠脾脏中铁元素的浓度为1.89×103 mg/kg，而给予MNPs的小鼠脾脏中铁元素的浓度显著增加至1.93×103 mg/kg。此外，还需要进行生化和免疫学参数的时间动力学分析，以确定其在生物给药中的适用性。©2022 Journal of Magnetics。

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来源期刊

Journal of Magnetics MATERIALS SCIENCE, MULTIDISCIPLINARY-PHYSICS, APPLIED

CiteScore

1.00

自引率

20.00%

发文量

审稿时长

2.3 months

期刊介绍： The JOURNAL OF MAGNETICS provides a forum for the discussion of original papers covering the magnetic theory, magnetic materials and their properties, magnetic recording materials and technology, spin electronics, and measurements and applications. The journal covers research papers, review letters, and notes.