Evaluation of different inoculation techniques on Pathogenecity of Sclerotium rolfsii

S. Mishra, P. Mishra, A. Mishra, Jaskaran Singh
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Abstract

Collar rot is one of the most destructive diseases of lentil. Yield losses can be attributed to fungal growth at collar region near soil line which leads to smaller and fewer seeds, premature ripening, shattered pods. The stem rot fungus overwinters as sclerotia in the soil, stubble or mixed with seed. Sclerotia can remain viable for five years or more when conditions are suitable sclerotia will germinate myceliogenically and directly infect plants near soil line. The present investigations were carried out the efficacy of various inoculation techniques of Sclerotium fungus in lentils. Maximum disease severity 44.5% were recorded in wheat inoculums method followed by agar disc method (40%) and the minimum disease severity 12% was observed in toothpick method.
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不同接种技术对罗氏菌核菌致病性的评价
扁豆领腐病是扁豆最具破坏性的病害之一。产量损失主要是由于真菌在近土线领区生长,导致种子小而少,早熟,豆荚破碎。茎腐菌以菌核形式在土壤中越冬,残茬或与种子混合。在条件适宜的情况下,菌核可以萌发菌丝体,直接侵染土壤线附近的植物。研究了不同菌核菌接种技术对小扁豆菌核菌的接种效果。小麦接种法最高检出率为44.5%,其次是琼脂盘法(40%),牙签法最低检出率为12%。
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