Evidence for a binding site on the sperm plasma membrane which recognizes the murine zona pellucida: A binding site on the sperm plasma membrane

G. R. Poirier, R. Robinson, R. Richardson, K. Hinds, D. Clayton
{"title":"Evidence for a binding site on the sperm plasma membrane which recognizes the murine zona pellucida: A binding site on the sperm plasma membrane","authors":"G. R. Poirier, R. Robinson, R. Richardson, K. Hinds, D. Clayton","doi":"10.1002/MRD.1120140307","DOIUrl":null,"url":null,"abstract":"Murine cauda epididymal sperm preincubated in either a modified Krebs-Ringer or M 199 solution bind to cumulus-free, zona pellucida-intact eggs. Pretreatment of such eggs with an affinity purified preparation of the seminal inhibitor binding component (acceptor), isolated from epididymal sperm, reduces in a concentration dependent manner, the number of sperm that bind. Treatment of cauda sperm, preincubated in either of the above two media, with the seminal inhibitor, also reduces the number of sperm able to bind. Incubation of cauda sperm in the Krebs-Ringer solution for up to 4 h does not affect their ability to bind the seminal inhibitor. Omission of bovine serum albumin from the preincubation medium results in a significant reduction in sperm binding. These data are interpreted to mean that the seminal inhibitor acceptor sites on the sperm surface of incubated sperm function in the in vitro binding to the zona pellucida.","PeriodicalId":12668,"journal":{"name":"Gamete Research","volume":"51 1","pages":"235-243"},"PeriodicalIF":0.0000,"publicationDate":"1986-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"37","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Gamete Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1002/MRD.1120140307","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 37

Abstract

Murine cauda epididymal sperm preincubated in either a modified Krebs-Ringer or M 199 solution bind to cumulus-free, zona pellucida-intact eggs. Pretreatment of such eggs with an affinity purified preparation of the seminal inhibitor binding component (acceptor), isolated from epididymal sperm, reduces in a concentration dependent manner, the number of sperm that bind. Treatment of cauda sperm, preincubated in either of the above two media, with the seminal inhibitor, also reduces the number of sperm able to bind. Incubation of cauda sperm in the Krebs-Ringer solution for up to 4 h does not affect their ability to bind the seminal inhibitor. Omission of bovine serum albumin from the preincubation medium results in a significant reduction in sperm binding. These data are interpreted to mean that the seminal inhibitor acceptor sites on the sperm surface of incubated sperm function in the in vitro binding to the zona pellucida.
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
精子质膜上识别小鼠透明带的结合位点的证据:精子质膜上的一个结合位点
在改良的krebs - ringger或m199溶液中预先孵育的小鼠尾附睾精子与无积云、透明带完整的卵子结合。用从附睾精子中分离出来的精液抑制剂结合成分(受体)的亲和纯化制剂预处理这些卵子,以浓度依赖的方式减少结合的精子数量。在上述两种培养基中的任何一种中,用精液抑制剂处理尾端精子,也会减少能够结合的精子数量。尾端精子在克雷布斯-林格溶液中孵育4小时不影响它们结合精子抑制剂的能力。从孵育前培养基中遗漏牛血清白蛋白导致精子结合显著减少。这些数据被解释为意味着孵育精子表面的精子抑制剂受体位点在体外与透明带结合中起作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
Genetic engineering of animals: An agricultural perspective, edited by J. Warren Evans and Alexander Hollaender; Plenum Press, New York, 1986, 328 pp. $49.50 Immunological evidence that a 305‐kilodalton vitelline envelope polypeptide isolated from sea urchin eggs is a sperm receptor Developmental capacity of mouse oocytes following maintenance of meiotic arrest in vitro Phosphoproteins are structural components of bull sperm outer dense fiber Effect of triton X‐100 on ultrastructure, reactivation, and motility characteristics of ram spermatozoa
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1