Development, characterization and anti-Mycobacterium tuberculosisactivity of solid lipid nanoparticles for oral Piper corcovadensisroots extract delivery

IF 0.7 4区医学 Q4 INTEGRATIVE & COMPLEMENTARY MEDICINE Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas Pub Date : 2023-11-30 DOI:10.37360/blacpma.23.22.6.55

Carla María Mariano Fernandez, Sirlene Adriana Kleinubing, T. K. Karam, F. Lorenzetti, C. T. Palomo, Byanca Pereira Moreira de Oliveira, Odinei Hess Gonçalves, Zilda Cristiani Gazim, Mariza Barion Romagnolo, K. R. Caleffi-Ferracioli, Regiane Bertin de Lima Scodro, Marli Miriam de Souza Lima, T. U. Nakamura, C. V. Nakamura, B. P. D. Filho

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Abstract

The present study thus aimed at the development and physicochemical characterization of solid lipid nanoparticles loaded with crude extract of Piper corcovadensisroots (SLN-CEPc) and chitosan-coated solid lipid nanoparticles loaded withcrude extract of P. corcovadensisroots (C-SLN-CEPc), as well as the determination of its antimycobacterial activity against Mycobacterium tuberculosisH37Rv, its cytotoxicity against the Vero cell line and evaluation in the hemolysis assay. Both formulations containing the encapsulated extract showed high encapsulation efficiency, formed by a monodispersed system with small and spherical particles, and there was no aggregation of particles. In the biological assays, SLN-CEPc and C-SLN-CEPc showed promising anti-M. tuberculosisactivity with a minimum inhibitory concentration (MIC) of 12.5 μg/mL, whereas the cytotoxic concentrations obtained at 50% (CC50) in Vero cells were 60.0 and 70.0 μg/mL, respectively. Therefore, nanoencapsulation showed satisfactory results, justifying its usage in the development of new products.

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口服胡椒根提取物固体脂质纳米颗粒的研制、表征和抗结核分枝杆菌活性

因此，本研究旨在开发装载胡椒根粗提取物的固体脂质纳米颗粒(SLN-CEPc)和装载胡椒根粗提取物的壳聚糖包被固体脂质纳米颗粒(C-SLN-CEPc)的物理化学特性，以及测定其对结核分枝杆菌ish37rv的抗细菌活性，对Vero细胞系的细胞毒性和溶血试验的评估。包封提取物的两种配方均表现出较高的包封效率，颗粒呈小球形单分散体系，无颗粒聚集现象。在生物实验中，SLN-CEPc和C-SLN-CEPc具有良好的抗m活性。最低抑菌浓度(MIC)为12.5 μg/mL，而50%时Vero细胞的细胞毒浓度(CC50)分别为60.0和70.0 μg/mL。因此，纳米胶囊化取得了令人满意的效果，证明了其在新产品开发中的应用。

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来源期刊

Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas PHARMACOLOGY & PHARMACY-

CiteScore

1.30

自引率

14.30%

发文量

审稿时长

6-12 weeks

期刊介绍： The Boletín Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas (BLACPMA), [Latin American and Caribbean Bulletin of Medicinal and Aromatic Plants]; currently edited by the publishing house MS-Editions, is a bi-monthly international publication that publishes original peerreviewed research in the field of medicinal and aromatic plants, with nearly 20 years of experience. BLACPMA is a scientific journal that publishes two types of articles: Reviews (only in English) and Original Articles (Spanish or English), its main lines of action being agronomy, anthropology and ethnobotany, industrial applications, botany, quality and standardization, ecology and biodiversity, pharmacology, phytochemistry, pharmacognosy, regulatory and legislative aspects. While all areas of medicinal plants are welcome and the experimental approaches used can be broad and interdisciplinary; other areas of research that are not mentioned depend on the Editorial Committee for their acceptance.