Inactivation of Human Rhinovirus due to Heat, UV Irradiation and Chemical Disinfectants

Abstract

To evaluate and understand inactivation of HRV under many physical conditions and chemical agents, HRV86 were selected to expose with temperature, ultraviolet light (UV), Sodium hypochlorite, Virkon S, Peracetic acid (PAA), Glutaraldehyde and Ethanolin, respectively. The inactivation of HRV was analyzed by infectivity of the viral strains on the HeLa cells. Our research found the rhinovirus was very sensitive to temperature changes. Viral infectivity thoroughly lost after HRV86 was treated at 60°C for 10 min or UV irradiation for 45 min or longer. Virus also was completely inactivated after exposure to sodium hypochlorite (0.1 g/L) beyond 10 min, glutaraldehyde (10 g/L) for 5 min, Virkon-S (5 g/L) for 10 min, PAA (3 g/L) for 2 min, or 75% alcohol for 5 min or longer. The results provided the essential information for prevention and intervention of common cold.