Leptospirosis: a problem for transfusion medicine?

M.A. Ribeiro , M.G. Cliquet , M.G.S. Santos
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引用次数: 3

Abstract

Leptospirosis is underdiagnosed owing to its non-specific presentation, especially in the early stages of the infection. In the present research, sera from 2368 healthy blood donors of a local blood bank were screened for the presence of leptospiral antibodies using IgM-Pk-ELISA, followed by the microscopic and macroscopic agglutination tests. Serum samples showing two positive and/or borderline results in these assays were confirmed by immunoblotting. The IgM-antibody profile was defined according to the following criteria: sera reactive to a diffuse band of molecular weight 14.8-22 kDa were considered leptospirosis positive, sera exhibiting no reactivity to this band were considered negative. Our results showed a prevalence of 1.01% positivity. There is evidence that transmission of Leptospira interrogans by blood transfusion is possible, but, to date there has been no documentation of transfusion-transmitted leptospirosis. Thus, according to the currently available information, there is no need for new bloodbanking procedures to identify possible carriers of L. interrogans.

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钩端螺旋体病:输血医学的问题?
由于其非特异性表现,特别是在感染的早期阶段,钩端螺旋体病的诊断不足。本研究采用igm -派克-酶联免疫吸附试验(elisa)对当地血库2368名健康献血者血清进行钩端螺旋体抗体筛查,并进行显微和宏观凝集试验。血清样品在这些检测中显示两个阳性和/或交界性结果,通过免疫印迹证实。igm抗体谱根据以下标准定义:对分子量为14.8-22 kDa的弥散带反应的血清为钩端螺旋体病阳性,对该带无反应的血清为阴性。结果显示阳性率为1.01%。有证据表明,有可能通过输血传播钩端螺旋体,但迄今为止没有关于输血传播的钩端螺旋体病的记录。因此,根据目前可获得的信息,不需要新的血库程序来识别可能的L.询盘菌携带者。
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