Elution Behavior of Carbohydrates for Core-Shell Ion-Exchange Resins with Different Degrees of Cross-Linking in Porous Shell Layer

Abstract

resin, suspension polymerization The St-80 resin high functionality, of the fully porous resin. carbohydrate analysis by HPLC using St-80 a Abstract A novel core-shell ion-exchange resin (St-80) having a 20:80 weight ratio of the monomer for the core and the shell was prepared for use in high-performance liquid chromatography (HPLC), and the effect of the degree of cross-linking (10–55%) of the porous shell on the separation of carbohydrates was examined. A mixed aqueous sample of inositol, glucose, fructose, and sucrose was reasonably separated under strong alkaline conditions (0.10 and 0.15 mol/L NaOH eluent) at flow rates of 0.3–0.7 mL/min. As the degree of cross-linking in the shell portion increased, the retention time of sucrose, which had the longest elution time, decreased. Meanwhile, the theoretical plate number nearly doubled. The retention times obtained for the high degrees of cross-linking (40% and 55%) in the porous shell were shorter than that of the fully porous resin. The theoretical plate number observed for these resins provided excellent resolution, similar to that of the fully porous resin.