ASSESSMENT OF CELLULASE COMPLEX SECRETORY CAPACITY OF TRICHODERMA STRAINS AND MORPHOLOGICAL AND MOLECULAR IDENTIFICATION OF THE ISOLATE WITH THE HIGHEST ENZYMATIC SECRETION CAPACITY

M. L. Castrillo, G. A. Bich, N. Amerio, María Daniela Rodríguez, P. Zapata, L. Villalba
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引用次数: 5

Abstract

The bioconversion of lignocellulosic biomass into monomeric sugars is a key economic difficulty hindering the profitable use of plant biomass as energy. The production of cellulase is a main factor in the cellulose hydrolysis. Among the main cellulase producers are the filamentous fungi. Therefore, many efforts have been made in obtaining new microorganisms with high cellulase secretion capacity. The cellulase secretory capacity of 28 isolates of Trichoderma was qualitatively and quantitatively evaluated. The detection of cellulolytic fungi was correlated with both Congo red and the dinitrosalicylic acid reagent methods. Based on qualitatively assays, sixteen of the isolates revealed carboxymethyl cellulose degradation ability, where the Trichoderma POS7 isolate showed the highest increase in filter paper activity, endo-1,4-β-glucanases and β-glucosidases activities (p<0.05) in a short incubation time. This isolate was molecularly identified as Trichoderma koningiopsis, based on internal transcribed spacer sequences. Our results provide new information and reveal new microorganism in the hydrolysis of cellulose material. The phylogenetic analysis revealed close positioning of T. koningiopsis clade with T. viride, T. viridescens and T. petersenii clades in a closely related group, in concordance with the current taxonomic classification of Trichoderma genus.
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木霉菌株纤维素酶复合物分泌能力评价及酶分泌能力最高菌株的形态和分子鉴定
木质纤维素生物质转化为单体糖是阻碍植物生物质作为能源的有效利用的关键经济困难。纤维素酶的产生是纤维素水解的主要因素。纤维素酶的主要生产者是丝状真菌。因此,人们为获得具有高纤维素酶分泌能力的新微生物做出了许多努力。对28株木霉的纤维素酶分泌能力进行了定性和定量评价。纤维素水解真菌的检测与刚果红法和二硝基水杨酸试剂法均具有相关性。定性分析结果表明,16株木霉POS7菌株在较短的培养时间内,滤纸活性、内切1,4-β-葡聚糖酶和β-葡萄糖苷酶活性均有显著提高(p<0.05)。根据内部转录的间隔序列,该分离物被分子鉴定为koningiopsis木霉。我们的研究结果为纤维素材料的水解提供了新的信息,揭示了新的微生物。系统发育分析表明,koningiopsis分支与木霉属(Trichoderma genus)、木霉属(viridescens)和木霉属(petersenii)分支定位相近,与木霉属的分类一致。
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