Random amplified polymorphic DNA markers detected in a segregating hybrid population of Solanum chacoense × S. phureja

Abstract

Reproducible random amplified polymorphic DNA (RAPD) patterns were obtained in two potato species (Solanum chacoense and S. phureja), one of their F1's, and its derived F2 population, by adjusting temperature profiles of the amplification process and template DNA concentrations (1 ng/μl reaction vol.). Although the number of amplified products and detectable differences between the two species increased with increasing GC content of the primer, 50% or 60% GC is recommended for maximizing scorable RAPDs in the F2 population. Using 82 primers, 589 RAPDs were detected between the parents, and 70% of them (409 RAPDs) in the F1 clone. The number of RAPDs reliably scored for their segregations in the F2 population was significantly lowered because of complicated RAPD patterns. Consequently, 22% of the RAPDs detected in the parents (129 RAPDs), an average of 1.57 RAPDs per primer, were obtained as genetic markers, which can be used for the construction of a genetic map for this particular population.