PARASITE FAUNA OF LABORATORY MICE

THEORY AND PRACTICE OF PARASITIC DISEASE CONTROL Pub Date : 2023-04-17 DOI:10.31016/978-5-6048555-6-0.2023.24.376-380

D. Polukhina, O. Panova, O. Kurnosova

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Abstract

Mice are widely used as laboratory animals. Diagnosis of endoparasites is a necessary condition: this is a culling indicator of animals for SPF (specific pathogen free) vivariums, and for conventional vivariums, it is the necessary information for planning and the possibility of using these animals in various tests. This article describes live-time diagnostics of endoparasites in laboratory mice in SPF vivariums and in open-type vivariums. For the study, individual samples of faeces and bedding were taken from laboratory mice. Direct fecal smears were examined and the flotation method was used. The work was carried out in 2021–2022 in the Laboratory of Biology and Biological Basis of Preventive Measures of the VNIIP – FSC VIEV. During this study, the following 180 samples were taken: 138 individual faecal samples, 34 samples combined with the bedding, and 18 bedding samples. The studied samples were found to contain Giardia muris and Tritrichomonas sp., nematodes Aspiculuris tetraptera and Syphacia obvelata, and cestode Rodentolepis nana. Mixed invasions were also recorded. The results showed the need for planning work with laboratory mice in vivariums.

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实验小鼠的寄生虫动物群

老鼠被广泛用作实验动物。内寄生虫的诊断是一个必要条件:这是SPF(无特定病原体)家养动物的扑杀指标，对于常规家养动物，这是规划和使用这些动物进行各种试验的可能性的必要信息。本文介绍了SPF型和开放式实验小鼠体内寄生虫的实时诊断方法。在这项研究中，从实验室老鼠身上提取了粪便和床上用品的样本。直接粪便涂片检查，采用浮选法。这项工作于2021-2022年在VNIIP - FSC VIEV生物学和预防措施生物学基础实验室进行。本研究共采集了以下180个样本:138个个体粪便样本，34个与床上用品结合的样本，18个床上用品样本。检出鼠贾第鞭毛虫、毛滴虫，四目弓形线虫、扁螺旋体线虫和小啮齿动物。混合入侵也有记录。结果表明，需要在实验室小鼠体内进行计划工作。

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THEORY AND PRACTICE OF PARASITIC DISEASE CONTROL

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