Antimicrobial Potential of Andrographis paniculata Conjugated Gold Nanoparticle

INNOSC Theranostics and Pharmacological Sciences Pub Date : 2019-07-17 DOI:10.26689/itps.v2i1.744

N. Razalli, S. Gopinath, F. Kasim, Ahmad Radi Wan Yaakub, P. Anbu

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引用次数: 2

Abstract

Background: Herbal extracts have been traditionally used as antibacterial agents, and different strategies have been proposed to enhance their antimicrobial activities. This research aimed to assess the antimicrobial potential of Andrographis paniculata conjugated gold nanoparticle (GNP) compounds against Escherichia coli and Bacillus subtilis.Methods: Herbal extract was conjugated with GNP through electrostatic interaction, and characterization was carried out by Fourier-transmission infrared spectroscopy, screening electron microscopy, and transmission electron microscopy and supported by energy-dispersive X-ray spectroscopy. Antibacterial activity of the herbal extract and GNP conjugation was evaluated by disc diffusion assay.Results: Chemical and morphological characterizations of the GNP and herbal extract conjugate revealed intactness of the GNP. In disc diffusion assay, the inhibition zone formed by these compounds was measured for both microorganisms, and they were in the range of 0.6-0.9 cm for the herbal extract. When the herbal extract conjugated with GNP, the zone was between 0.8 and 1.1 cm.Conclusion: This study demonstrated the potentiality of GNP to carry the antibacterial compounds from the herbal extract.

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穿心莲共轭金纳米颗粒的抗菌潜力

背景:传统上，草药提取物一直被用作抗菌药物，人们提出了不同的策略来提高其抗菌活性。本研究旨在评价穿心莲偶联金纳米颗粒(GNP)化合物对大肠杆菌和枯草芽孢杆菌的抑菌潜力。方法:通过静电相互作用将中药提取物与GNP偶联，采用傅里叶红外光谱、筛选电镜和透射电镜对其进行表征，并采用能量色散x射线光谱进行表征。采用圆盘扩散法对中药提取物和GNP偶联物进行抑菌活性评价。结果:GNP和草药提取物偶联物的化学和形态特征显示GNP的完整性。在圆盘扩散法中，测定了这些化合物对两种微生物形成的抑制带，对草药提取物的抑制带在0.6 ~ 0.9 cm范围内。当与GNP偶联时，该区域在0.8 ~ 1.1 cm之间。结论:本研究证明了国产GNP具有携带中药提取物中抗菌化合物的潜力。

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INNOSC Theranostics and Pharmacological Sciences

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