Functional characterization of L-tryptophan transport across mammalian cornea

M. Rathore, V. Gupta
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引用次数: 2

Abstract

In last few years transporter targeted drug delivery has drawn attention of research to identify and explore various nutrient transport systems including amino acid transporters for better drug delivery. The aim of present research work is to investigate the transport characteristics of L-tryptophan (L-try) across goat cornea. Transport of L-try was investigated using a glass diffusion cell for effect of concentration, pH, presence of other amino acids or metabolic inhibitor or dipeptide and tripeptide. The amount of L-try transported increased as the pH of L-try aqueous solution increased from 5 to 9. Inhibition was observed in L-try transport in absence of sodium ions where L-try solution was made isotonic with dextrose. Amino acids like L-histidine, L-arginine, L-lysine (cationic), L-glutamic acid, L-aspartic acid (anionic), glycine and L-proline (neutral) inhibited the L-try transport as compared to control (L-try alone). In presence of sodium azide and Ouabain the inhibition in L-try transport across goat cornea was observed while no marked inhibition was observed on L-try transport across goat cornea in presence of aspartame and glutathione. The L-try transport was favored up to concentration 1% w/v and at higher pH in presence of sodium ions through excised goat cornea. Functional presence of a sodium dependent L-try transport system as inhibited by ouabain having affinity to cationic and neutral amino acid is evident on goat cornea. Keywords: Cornea; Amino acid; Tryptophan; Transport
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l -色氨酸在哺乳动物角膜间转运的功能表征
近年来,以转运体为靶点的药物递送研究已引起人们的关注,以识别和探索包括氨基酸转运体在内的各种营养转运系统,以更好地给药。本研究的目的是研究l -色氨酸(L-try)在山羊角膜中的转运特性。利用玻璃扩散池研究了L-try的转运,考察了浓度、pH、其他氨基酸或代谢抑制剂或二肽和三肽的存在对L-try转运的影响。L-try的输运量随着L-try水溶液pH从5增加到9而增加。在没有钠离子的情况下,将L-try溶液与葡萄糖制成等渗溶液,观察到L-try转运受到抑制。与对照组相比,l -组氨酸、l -精氨酸、l -赖氨酸(阳离子)、l -谷氨酸、l -天冬氨酸(阴离子)、甘氨酸和l -脯氨酸(中性)等氨基酸抑制了L-try的转运。叠氮化钠和谷胱甘肽对山羊角膜l -试体转运有抑制作用,而阿斯巴甜和谷胱甘肽对山羊角膜l -试体转运无明显抑制作用。当钠离子存在时,L-try在1% w/v的浓度和更高的pH下通过山羊切除的角膜进行转运。在山羊角膜上,钠依赖的L-try运输系统的功能存在是明显的,它被具有阳离子和中性氨基酸亲和力的瓦沙因所抑制。关键词:角膜;氨基酸;色氨酸;运输
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