Evaluation of binding affinity of synthesized coumarin derivative on single-stranded DNA by spectroscopic methods

Abstract

3-(tetrazole-5-yL) coumarin, Single-stranded DNA, Fluorescence spectroscopy, Circular dichroism spectroscopy Aim: According to the importance of coumarin derivatives as an effective medication on cancer cells and various other therapeutic effects, in this study we investigated the effect of a new derivative of coumarin named 3(tetrazol5-yl) coumarin on single-stranded DNA by different spectroscopic methods in solution. Material and Methods: The present study has investigated the effect of 3(tetrazol-5-il) coumarin on single-stranded DNA in vitro. The findings demonstrates that the rate of single strand DNA absorption enhances by interaction with 3-(tetrazol-5-yl) coumarin at 210 and 260 nm. The fluorescence intensity of single-stranded DNA increases in a concentrationdependent of 3(tetrazol-5-yl) coumarin, indicating the binding of 3(tetrazol5-yl) coumarin to the chromophores in single-stranded DNA. Results: Binding of 3(tetrazol-5-yl) coumarin to single-stranded DNA causes a significant increase in ellipticity in circular dichroism of DNA molecules in the regions of 220 and 275 nm which is more positive at 245 nm. The results indicate a stronger binding of 3(tetrazol-5-l) coumarin to single-stranded DNA, which may be due to the fact that single-stranded DNA may be more available during replication. Conclusion: The results obtained from the effect of 3(tetrazol-5-yl) coumarin on single-stranded DNA can provide valuable information to design medications by coumarin derivatives which have more anti-tumor effect and less side effects. هرود ،تفاب و لولس 13 هرامش ، 1 لاس ، 1401 تاحفص ، 23 ات 33