デオキシニバレノールがS. cerevisiae PTC1変異株に及ぼす遺伝子発現変化の解析

IF 0.4 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Chem-Bio Informatics Journal Pub Date : 2011-01-01 DOI:10.1273/CBIJ.11.41
忠 鈴木, 岩橋 由美子
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Abstract

Deoxynivalenol (DON) is a secondary metabolite that is generated by Fusarium species, which seriously affects both humans and livestock. Protein synthesis inhibition and ribotoxic stress, caused by induction of the mitogen activated protein kinase (MAPK) cascade, are thought to be responsible for the majority of DON toxicity. However, as DNA damage has also been reported, it is necessary to clarify all sources of toxicity. In this study, we conducted a DON exposure test using the PTC1 yeast mutant with disrupted MAPK-related genes, and observed gene expression changes using DNA microarray analysis. Our results indicated changes in the expression of genes associated with protein synthesis inhibition, as well as with DNA damage. At the same time, genes related to the synthesis of folic acid, a coenzyme in DNA synthesis, were inhibited. To complement the dysfunction of these genes, the growth media was supplemented with folic acid. As a result, the recovery of growth was confirmed, although it was a consistent effect and it did not reflect differences in susceptibility to DON toxicity.
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脱氧戊烯醇对S. cerevisiae PTC1变异株的基因表达变化分析
脱氧雪腐镰刀菌醇(脱氧雪腐镰刀菌醇,DON)是镰刀菌产生的次生代谢物,严重影响人类和牲畜。有丝分裂原活化蛋白激酶(MAPK)级联反应引起的蛋白质合成抑制和核素毒性应激被认为是造成DON毒性的主要原因。然而,由于DNA损伤也有报道,有必要澄清所有毒性来源。在本研究中,我们使用mapk相关基因被破坏的PTC1酵母突变体进行DON暴露试验,并使用DNA微阵列分析观察基因表达变化。我们的研究结果表明,与蛋白质合成抑制以及DNA损伤相关的基因表达发生了变化。与此同时,与DNA合成中的辅酶叶酸合成相关的基因被抑制。为了弥补这些基因的功能障碍,在生长培养基中添加叶酸。结果证实了生长的恢复,尽管这是一个一致的效果,并没有反映出对DON毒性敏感性的差异。
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来源期刊
Chem-Bio Informatics Journal
Chem-Bio Informatics Journal BIOCHEMISTRY & MOLECULAR BIOLOGY-
CiteScore
0.60
自引率
0.00%
发文量
8
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