L. T. Quyen, Nguyễn Thị Minh Thu, Le Thi Lan Phuong, N. Trang
{"title":"Comparison of the SSSperm testing kitwith the Halosperm testing kit in an analysis of sperm DNA fragmentation","authors":"L. T. Quyen, Nguyễn Thị Minh Thu, Le Thi Lan Phuong, N. Trang","doi":"10.31276/vjste.62(2).71-76","DOIUrl":null,"url":null,"abstract":": The objectives of this work were to accurately evaluate testing kits that analyse sperm DNA fragmentation in infertile men and to provide a comparison of an improved testing kit (SSSperm testing kit) to the existing Halosperm testing kit in an analysis of sperm DNA fragmentation. A cross-sectional study was conducted on 300 semen samples from infertile men with a sperm concentration ≥1 million/ml using the Bland-Altman, T-test, and Pearson test for statistical study. The SSSperm testing kit had a coefficient of variation of CV%=2.26%<5% and t tn =0.97<t c =2. The two methods have similar DNA fragmentation index (DFI) results (r=0.995; p<0.001). The difference between the results of the two kits was not statistically significant (p=0.236>0.05). In conclusion, the SSSperm testing kit for the analysis of sperm DNA fragmentation is qualified as determined from quantitative tests, and the SSSperm testing kit is equivalent to the Halosperm testing kit.","PeriodicalId":23548,"journal":{"name":"Vietnam Journal of Science, Technology and Engineering","volume":"63 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2020-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Vietnam Journal of Science, Technology and Engineering","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.31276/vjste.62(2).71-76","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
: The objectives of this work were to accurately evaluate testing kits that analyse sperm DNA fragmentation in infertile men and to provide a comparison of an improved testing kit (SSSperm testing kit) to the existing Halosperm testing kit in an analysis of sperm DNA fragmentation. A cross-sectional study was conducted on 300 semen samples from infertile men with a sperm concentration ≥1 million/ml using the Bland-Altman, T-test, and Pearson test for statistical study. The SSSperm testing kit had a coefficient of variation of CV%=2.26%<5% and t tn =0.970.05). In conclusion, the SSSperm testing kit for the analysis of sperm DNA fragmentation is qualified as determined from quantitative tests, and the SSSperm testing kit is equivalent to the Halosperm testing kit.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
