Green Spectrofluorimetric Technique for the Determination of Vildagliptin Through Quenching Lanthanide Luminescence in Pure, Pharmaceutical Dosage Form and Spiked Plasma

Abstract

Objectives: The objective of the proposed method is to develop a validated sensitive, selective, rapid, and low-cost spectrofluorimetric technique for the estimation of Vildagliptin depending on its quenching effect on the fluorescence intensity of Terbium (Tb). Methods: The proposed method involves quantitative fluorescence quenching of terbium measured at 477 nm after excitation at 239 nm. Various experimental conditions were investigated as pH, time of reaction, order of mixing of reagents, concentrations of Tb and buffer. Results: Under optimum conditions, good linearity was obtained within the range 20.0-3000.0 ng/mL with correlation coefficients of 0.9997. LOD and LOQ were 1.8 ng/mL and 19.2 ng/mL, respectively. Conclusion: The proposed method accomplished greener parameters; accordingly, it is a green choice for the determination of Vildagliptin in bulk, pharmaceutical dosage form, and spiked plasma.