PERAN PROTEIN HEMAGLUTININ PILI Streptococcus pneumoniae 54 kDa SEBAGAI ADHESIN

Abstract

Received: January, 20, 2020 Revised: May, 6, 2020 Available online: August, 2020 Introduction: Pili has an adhesin that plays a role in the adhesion process in order to infect the host cell. Research shown that the 54 kDa S. pneumoniae pili protein is a hemagglutinin. This study was to examine whether the 54 kDa of S. pneumoniae pili protein also act as an adhesin. Method: S. pneumoniae was isolated using a pili cutter. The protein molecular weight was analyzed using SDS-PAGE. Protein with a molecular weight of 54 kDa was isolated to produce protein solution. Adhesion test was carried out on a protein solution with multilevel concentrations to determine the adhesion index. Result: Pearson correlation test obtained p-value of 0.036 and the correlation coefficient R = -0.840, these results indicate that the two variables have a significant negative relationship. Regression analysis showed R2 0,997, mean that 99.7% of the concentration of protein S. pneumoniae 54 kDa influenced the adhesion index. Conclusion: Therefore, it can be concluded that the hemagglutinin protein S. pneumoniae 54 kDa is an adhesin protein. A B S T R A K Latar Belakang: Pili memiliki protein adhesin yang berperan dalam proses adhesi untuk menginfeksi sel hospes. Penelitian sebelumnya menunjukkan bahwa protein pili S. pneumoniae 54 kDa merupakan protein hemaglutinin. Tujuan penelitian ini adalah menguji peran protein hemaglutinin pili S. pneumoniae 54 kDa sebagai adhesin. Metode: Pili S. pneumoniae diisolasi menggunakan alat pili cutter. Hasil potongan pili dilakukan SDS-PAGE untuk mengidentifikasi berat molekul proteinnya. Protein berat molekul 54 kDa diisolasi sehingga menghasilkan larutan protein. Larutan protein diuji adhesi dengan konsentrasi bertingkat untuk mengetahui indeks adhesi. Hasil: Uji korelasi Pearson diperoleh nilai p-value 0,036 (p < 0,05) dan koefisien korelasi R= -0,840. Hasil ini menunjukkan bahwa kedua variabel memiliki hubungan yang signifikan, sangat kuat dengan arah hubungan negatif. Analisis regresi didapatkan R2 0,997, artinya 99,7 % konsentrasi protein pili 54 kDa S. pneumoniae mempengaruhi indeks adhesi. Kesimpulan: protein hemaglutinin pili 54 kDa S. pneumoniae merupakan protein adhesin