Evaluation and comparison of the antimicrobial activity of herbal extracts with the standard irrigant against Enterococcus faecalis

Jenny Atom, N. Devi, Nikita Kangabam, Bebika Thoudam, Ronel Lairenlakpam, N. Singh
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Abstract

Background: Numerous actions to diminish the number of microorganisms in the root canal system comprise the usage of a variety of functional techniques, irrigation methods, and intracanal medications. Irrigation goes hand in hand with resources to help remove pulp tissue and/or microorganisms. Purpose: The intent of this trial was to assess as well as compare the antimicrobial activity of herbal irrigant with normal irrigant (2.5% sodium hypochlorite [NaOCl]) against Enterococcus faecalis to test its effectiveness as root irrigation water. Materials and Methods: The culture was elevated overnight at 37°C in brain heart infusion (BHI) broth followed by testing microbial growth for changes in turbidity for 24 h. BHI broth comprising E. faecalis is vaccinated on a BHI agar plate using a grass planting method utilizing a sterile glass dispenser. Five test herbal discs (Curcuma longa [CT]-turmeric, Azadirachta indica [AI], MI, Propolis [P], and Triphala [T]); in addition to two control discs (ethanol as well as NaOCl) positioned on a custom plate. Results: The average blocking area with a drop-down program for each drug was observed to be: NaOCl (24.0 ± 1.74) >P (14.6 ± 1.84) > AI (09.5 ± 1.72) > T (06.4 ± 0.83) > CT (04.3 ± 04.1± ±0.83).) > Morinda citrifolia (03.2 ± 0.30) > ethanol (00.1 ± 0.01). Conclusions: P and AI have shown assuring outcomes in the completion of E. faecalis. Nevertheless, their work has been observed to be under NaOCl.
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中药提取物与标准冲洗液对粪肠球菌抗菌活性的评价与比较
背景:减少根管系统内微生物数量的许多措施包括使用各种功能技术、冲洗方法和管内药物。灌溉与资源齐头并进,帮助去除牙髓组织和/或微生物。目的:本试验的目的是评估和比较草药灌溉水与普通灌溉水(2.5%次氯酸钠[NaOCl])对粪肠球菌的抗菌活性,以测试其作为根灌溉水的有效性。材料和方法:将培养物在37°C的脑心输注(BHI)肉汤中升高过夜,然后检测微生物生长的浊度变化24小时。含有粪肠杆菌的BHI肉汤在BHI琼脂板上接种,使用无菌玻璃分配器采用植草方法。五种试验草药片(姜黄[CT]-姜黄,印楝[AI], MI,蜂胶[P]和Triphala [T]);除了两个控制盘(乙醇以及NaOCl)定位在一个定制的板。结果:各药物的平均阻断面积为:NaOCl(24.0±1.74)>P(14.6±1.84)> AI(09.5±1.72)> T(06.4±0.83)> CT(04.3±04.1±±0.83)>桑叶(03.2±0.30)>乙醇(00.1±0.01)。结论:P和AI在完成粪肠杆菌治疗中显示出令人满意的结果。然而,据观察,他们的工作是在NaOCl的指导下进行的。
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