Infiltration by Streptococcus mutans in provisional crowns fixed by two types of cementing agents – pilot double-blind randomized clinical trial

A. Rego, Vanessa Anielly Cruz Moura, J. Prado, F. Alexandrino, R. R. Regis, K. M. D. O. Pontes
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Abstract

Objective : When provisional acrylic crowns are used for a long time, they become more susceptible to marginal leakage by cariogenic bacteria. The objectives of this pilot clinical study were to compare cement based on zinc oxide-eugenol and calcium hydroxide by contamination with Streptococcus mutans, and calculate the sample size for the continuation of this study. Methods : Individuals receiving provisional crowns and following inclusion/exclusion criteria, were randomly distributed into 2 groups: zinc oxide-eugenol (n=8); calcium hydroxide (n=9). The temporary crowns were made by a blind researcher and cemented by another. Patients were also blinded by the cement used inside their crowns. After 2 months, a cement sample from the crowns’ peripheral inner face was collected, placed in a tube containing 1 mL of sterile saline, serially diluted, plated on Mitis Salivarius Bacitracin agar, and incubated for 48 hours. Colony-forming units (CFU/mL) were counted. A statistical power analysis was performed to calculate sample size (1-β=80%) and the Mann Whitney test to compare both cements (α=0.05). Results : Both cements were contaminated with S. mutans, with an average of 166.6 x 102 CFU/mL for calcium hydroxide and 435.3 x 102 CFU/mL for zinc oxide-eugenol, with no significant difference (p=0.311). The sample size calculated for this study was 36 per group. Conclusion : This pilot study suggests that there is important contamination inside provisional crowns used for two months, independent of the cement. The continuation of this study is needed, with a bigger sample size, to enable a comparison between the cements.
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变形链球菌在两种胶结剂固定的临时冠中的浸润——先导双盲随机临床试验
目的:长期使用临时丙烯牙冠,易因牙源性细菌而发生牙缘渗漏。本初步临床研究的目的是比较氧化锌-丁香酚水泥和氢氧化钙水泥受变形链球菌污染的情况,并计算本研究继续进行的样本量。方法:接受临时冠治疗并符合纳入/排除标准的患者随机分为2组:氧化锌-丁香酚组(n=8);氢氧化钙(n=9)。这些临时的牙冠是由一位盲人研究人员制作的,并由另一位盲人进行粘接。患者还会因冠内使用的水泥而失明。2个月后,收集牙冠周围内表面的水泥样品,放入装有1 mL无菌生理盐水的管中,连续稀释,涂于Mitis Salivarius Bacitracin琼脂上,孵育48小时。计数菌落形成单位(CFU/mL)。采用统计功效分析计算样本量(1-β=80%), Mann Whitney检验比较两种水泥(α=0.05)。结果:两种骨水泥均存在变形链球菌污染,氢氧化钙平均为166.6 × 102 CFU/mL,氧化锌-丁香酚平均为435.3 × 102 CFU/mL,差异无统计学意义(p=0.311)。本研究计算的样本量为每组36人。结论:这项初步研究表明,使用两个月的临时冠内存在重要的污染,独立于水泥。这项研究需要继续进行,样本量更大,以便对水泥进行比较。
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