CHANGES IN MEMBRANE FLUIDITY DURING THE MICELLE FORMATION DETERMINE THE EFFICIENCY OF THE SOLUBILIZATION OF MUSCARINIC RECEPTORS

S. Kopanchuk, A. Rinken
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引用次数: 7

Abstract

The influence of various concentrations of 15 detergents at different temperatures on the fluidity of membranes was analysed by fluorescence anisotropy of inserted 1,6-diphenyl-1,3,5- hexatriene in rat cortical and Sf9 cell membranes. Comparison of the obtained data with the abilities of the detergents to solubilize muscarinic receptor subtypes indicated that not only the fluidity of the micelles formed but also the micelle formation process, the lipid/protein ratio, and presence of sterols have significant influence on the efficiency of the solubilization of mAChR. Digitonin seems to exploit efficiently all these factors by its biphasic modulatory effect on membrane fluidity during the receptor solubilization.
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胶束形成过程中膜流动性的变化决定了毒蕈碱受体的增溶效率
通过大鼠皮质和Sf9细胞膜中插入1,6-二苯基-1,3,5-六三烯的荧光各向异性,分析了15种洗涤剂在不同温度下不同浓度对膜流动性的影响。将所得数据与洗涤剂对毒蕈碱受体的增溶能力进行比较,结果表明,除了形成胶束的流动性外,胶束形成过程、脂蛋白比和甾醇的存在对mAChR的增溶效率也有显著影响。洋地黄苷在受体溶解过程中对膜流动性的双相调节作用似乎有效地利用了所有这些因素。
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