Yielding and Growing of Adipose Stem Cell Harvested from the Superficial and Deep Abdominal Subcutaneous Fat: A Case

Abstract

A 60 year old woman was enrolled in our study. She was 151 cm tall, weighed 73 kg and had high body mass index (33.19 kg/m2). Adipose tissue specimens were taken from her abdomen by surgical excision. ASCs were isolated in primary culture and cell counts were performed by using an automated cell counter (LUNA™). And 5 × 103 ASCs were seeded into DMEM tissue culture flasks and were cultured for 16 days (n=3). Lastly, self-renewal capacity was demonstrated by colony-forming unit fibroblast (CFUF) assays (n=3).