Modeling the female hypogonadism by means of ovarian ischemization and its morphofunctional justification

Abstract

The present study is devoted to the morphofunctional substantiation of the model of female hypogonadism, which is important for the subsequent evaluation of the effectiveness of its targeted pharmacological correction, as well as to elucidate the resistance of ovarian endocrine cells to damage as a result of experiencing a single acute ischemic effect. In three groups of adult female rats under anesthesia, the arteries of the right and left ovaries were ligated for 30, 45, and 60 min (4 rats in each group). After the operation, the wound was sutured. Sham-operated rats (4 individuals) served as controls. After 7 days, the results were analyzed. The blood levels of sex steroid hormones (17-estradiol, progesterone) and gonadotropins (FSH and LH) were determined by enzyme-linked immunosorbent assay. In the meridional histological sections of the mature tertiary follicle and the corpus luteum of the right and left ovaries, after survey staining, the number and percentage of viable and dead endocrinocytes were counted, and the area of viable endocrine cells was determined. The significance of differences in the median, upper and lower quartiles of the compared parameters was determined using the nonparametric MannWhitney test. It has been established that a single acute ovarian ischemia induces massive death and degenerative changes in a significant part of the viable endocrine cells of the mature tertiary follicle and corpus luteum, most pronounced with a one-hour bilateral occlusion of the ovarian vessels. These quantitative and structural changes in interstitial endocrine cells and ovarian luteocytes cause a significant decrease in the production of sex steroids, which, in turn, causes hyperproduction of the corresponding pituitary tropins. Morphological changes in both types of ovarian endocrine cells in this model of acute single ovarian ischemia are highly sensitive; the use of a number of morphometric parameters of endocrinocytes in combination with the determination of the concentration of peripheral and regulatory homones will allow using this model to assess the effectiveness of the correction of female hypogonadism with various pharmacological preparations.