Comparison of Antivenom potential of chicken Egg yolk Antibodies Generated against Bentonite and Adjuvant coated Echis carinatus venom

The Internet Journal of Pharmacology Pub Date : 2008-12-31 DOI:10.5580/1244

S. Meenatchisundaram, R. Selvakumaran, G. Parameswari, A. Michael

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引用次数: 2

Abstract

The Chicken egg yolk antibodies generated against Bentonite and Adjuvant (FCA) coated Echis carinatus venom was evaluated for their anti-venom potential. Antibodies are extracted from immunized chicken egg yolk by the method described by Polson et al.(1980) and further purified by DEAE cellulose ion exchange column chromatography, which gave pure (180 - 200KDa) specific antibodies against venom. Inhibition of lethal, edema, procoagulant, and phospholipase A2 activities of Echis carinatus venom was determined. We found that 1.27mg of IgY (generated against FCA coated venom) and 1.33mg of IgY (generated against Bentonite coated venom) was able to completely neutralize the lethal activity (2LD 50) of Echis carinatus venom. Both chicken egg yolk antibodies effectively inhibited procoagulant activity and partially inhibited the Edema forming activity. IgY generated using Adjuvant (FCA) coated venoms showed very high anti-phospholipase A2 activity. In general, the results demonstrated the effectiveness of chicken egg yolk antibodies raised against adjuvant and bentonite coated venoms in neutralizing various pharamacological effects of Echis carinatusvenom.Keywords: Venoms, FCA, Bentonite, Chicken antibodies (IgY), PLA 2

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鸡卵黄抗膨润土和佐剂包被胭脂鱼毒抗体抗蛇毒潜能的比较

研究了鸡卵黄抗体对膨润土和佐剂(FCA)包被胭脂鱼毒的抗毒能力。用Polson等人(1980)的方法从免疫后的蛋黄中提取抗体，再用DEAE纤维素离子交换柱层析纯化，得到纯的(180 - 200KDa)特异性抗蛇毒抗体。测定了鲫鱼毒液对致死性、水肿性、促凝性和磷脂酶A2活性的抑制作用。我们发现，1.27mg的IgY(对FCA包被毒液产生)和1.33mg的IgY(对膨润土包被毒液产生)能够完全中和Echis carinatus毒液的致死活性(2ld50)。两种鸡蛋黄抗体均能有效抑制促凝剂活性，部分抑制水肿形成活性。用佐剂(FCA)包被毒液生成的卵磷脂具有很高的抗磷脂酶A2活性。综上所述，针对佐剂和膨润土包被毒液制备的蛋黄抗体能有效中和蛇腹毒的多种药理作用。关键词:毒液，FCA，膨润土，鸡抗体，PLA 2

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The Internet Journal of Pharmacology

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