Fabiola Islas, Andrea Sabido, Juan-Carlos Sigala, Alvaro R Lara
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引用次数: 0
Abstract
Plasmid DNA manufacture is an essential step to produce gene therapy agents and next-generation vaccines. However, little attention has been paid toward developing alternative replicons that can be coupled with large-scale production conditions. Our results demonstrate that the miniR1 replicon can be efficiently induced by oxygen limitation when a copy of the regulatory protein RepA under control of a microaerobic promoter is used. The results are potentially attractive for industrial applications.
期刊介绍：
The Journal of Hydraulic Research (JHR) is the flagship journal of the International Association for Hydro-Environment Engineering and Research (IAHR). It publishes research papers in theoretical, experimental and computational hydraulics and fluid mechanics, particularly relating to rivers, lakes, estuaries, coasts, constructed waterways, and some internal flows such as pipe flows. To reflect current tendencies in water research, outcomes of interdisciplinary hydro-environment studies with a strong fluid mechanical component are especially invited. Although the preference is given to the fundamental issues, the papers focusing on important unconventional or emerging applications of broad interest are also welcome.
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