In-Vitro Wound Healing, Antioxidants, Antibacterial and Cytotoxic Activity of Probiotic Bacteria Isolated from Spoiled Curd

Abstract

Lactic acid bacteria (LAB) isolated from curd has emerged as a welcome source of antimicrobials and therapeutics and is accepted as probiotics. Probiotics have been associated with improved healing of intestinal ulcers and healing of infected cutaneous wounds. Therefore this current study was undertaken to assess the antibacterial, antioxidant, minimum inhibitory concentration and cytotoxicity efficacy of Lactobacilli isolated from spoiled curd sample. The bacteria was isolated using serial dilution method and the antagonistic properties of these isolates against Bacillus subtilis, Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa were examined using agar well diffusion method. Amongst the 8 isolates, C3 exhibited the highest antibacterial activity against all the pathogens. It was then tested for antioxidant property using DPPH assay and also found wound healing property using 3T3 cell line. This review points to a new perspective and therapeutic potential of beneficial probiotic species as a safe alternative approach for treatment of patients affected by cutaneous wounds.