Optimization of ultrasonic-assisted extraction of bioactive compounds from Bupleuri Radix by response surface methodology and HPLC analysis

Jia-hong Sun, Xuejiao Li, Zhuo Qu, Shengjie Dong, H Zhao
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Abstract

A simple and efficient ultrasonic-assisted extraction of bioactive compounds from raw Bupleuri Radix and vinegar-baked Bupleuri Radix was developed and optimized by using quantitative analysis of multi-components by a single-marker combined with response surface methodology approaches. The quantitative analysis of multi-components by a single-marker analytical method based on high-performance liquid chromatography coupled with a photodiode array detector was established and applied to determine the contents of six bioactive compounds in raw Bupleuri Radix and vinegar-baked Bupleuri Radix. In order to maximize extraction of six bioactive compounds in raw Bupleuri Radix and vinegar-baked Bupleuri Radix, the ultrasonic-assisted extraction conditions were obtained by the response surface methodology coupled with Box–Behnken design. The conditions of ultrasonic-assisted extraction were found to be effective for the extraction of bioactive compounds from raw Bupleuri Radix and vinegar-baked Bupleuri Radix. The test results showed that the difference in saikosaponins contents in these samples was obvious; a quantitative analysis of saikosaponins will contribute significantly to improving the quality control of raw Bupleuri Radix and vinegar-baked Bupleuri Radix. In conclusion, the designed protocol is suitable for the quality control of raw Bupleuri Radix and vinegar-baked Bupleuri Radix.
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响应面法和高效液相色谱法优化超声辅助提取柴胡中活性物质的工艺条件
采用单标记法结合响应面法对多组分进行定量分析,建立了一种简单高效的超声辅助提取柴胡和醋烤柴胡中活性成分的方法。建立了基于高效液相色谱-光电二极管阵列检测器的多组分定量分析方法,并应用该方法测定了生柴胡和醋烤柴胡中6种生物活性成分的含量。为了最大限度地提取生柴胡和醋烤柴胡中的6种活性物质,采用响应面法结合Box-Behnken设计确定了超声辅助提取条件。研究了超声辅助提取条件对柴胡生料和醋烤柴胡中活性成分的提取效果较好。检测结果表明,这些样品中柴胡皂苷含量差异明显;柴胡皂苷的定量分析将有助于提高柴胡生料和醋烤柴胡的质量控制。综上所述,所设计的工艺方案适用于生柴胡和醋烤柴胡的质量控制。
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