Assessment of the Larvicidal Potency of Bacillus thuringiensis Against the Moth of Spoladea recurvalis on Amaranths Plant

R. Muhammed, O. Yahaya, D. Machido
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Abstract

The control of Spoladeare curvalis, a major pest of amaranth plant, has been through the use of synthetic pesticides which have been reported to have detrimental effects. This study was aimed at assessing the larvicidal potency of B. thuringiensis against the larvae of Spoladea recurvalis on amaranth plant. The assessment of the larvicidal potency of B. thuringiensis isolates from cow range land and refuse dump sites in Zaria metropolis habouringCry1 gene (CR2, CR4, RD1) was carried out by spraying spore crystal mixture of B. thuringiensis isolates on amaranth plants infested with Spoladea recurvalis larvae. The mortality of S. recurvalis larvae, under a screen house were assessed after 24, 48 and 72hours of exposure to the spore crystal mixture of B. thuringiensis isolates. Spore crystal mixtures of B. thuringiensis isolates were effective in causing larvae mortality with more than 60% mean mortality rate of S. recurvalis larvae after 72hours of exposure. The mortality rate of Spoladea rescurvalis increased with increase in exposure period. Spore crystal mixture of Bacillus thuringiensis isolates should be considered as an alternative to synthetic pesticides for the control of Spoladea recurvalis larvae on amaranth plant. Keywords: Bacillus thuringiensis; spore; larvae; amaranth; larvicidal.
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苏云金芽孢杆菌对苋菜褐飞蛾的杀幼虫力评价
对苋属植物的一种主要害虫——曲斑孢虫的控制一直是通过使用合成杀虫剂来进行的,据报道,这种杀虫剂具有有害的效果。研究了苏云金芽孢杆菌对苋菜反刍孢子虫幼虫的杀虫效果。通过将含有cry1基因(CR2、CR4、RD1)的苏云金芽孢杆菌分离株孢子结晶混合物喷施在有反叶孢子虫幼虫侵染的苋菜植株上,评价了该分离株的杀虫效果。分别于苏云金芽孢杆菌孢子结晶混合物暴露24、48和72小时后,在纱窗下测定了苏云金芽孢杆菌幼虫的死亡率。苏云金芽孢杆菌分离株的孢子晶体混合物对苏云金芽孢杆菌幼虫有明显的杀伤作用,暴露72小时后,平均死亡率超过60%。随着暴露时间的延长,黑穗虫的死亡率呈上升趋势。苏云金芽孢杆菌分离株的孢子结晶混合物可作为合成农药的替代品,用于防治苋菜上的反曲孢子蝇幼虫。关键词:苏云金芽孢杆菌;孢子;幼虫;苋菜;杀灭幼虫。
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