{"title":"Methods to spontaneously generate three dimensionally arrayed microdroplets triggered by capillarity for bioassays and bioengineering.","authors":"Hiroki Yasuga","doi":"10.2142/biophysico.bppb-v20.0029","DOIUrl":null,"url":null,"abstract":"<p><p>Herein, I review our recent work toward developing methods for generating three-dimensional (3D) droplet arrays driven by capillarity. Microdroplet array-based systems are useful for bioassays and bioengineering because they require only small amounts of samples and reagents and provide the high throughput. Various methods have been developed for preparing droplet arrays, among which methods based on capillarity have attracted considerable attention owing to their simplicity. I and collaborators have developed such methods based on capillary flow, including a method for preparing droplet arrays via oil-water replacement. We recently proposed our own concept of \"fluid-fluid interfacial energy driven 3D structure emergence in a micropillar scaffold (FLUID3EAMS)\" and its application. FLUID3EAMS allows a 3D droplet (or hydrogel bead) array to be generated in a micropillar scaffold by passing a fluid-fluid interface through the scaffold. This approach is useful for applications requiring ordered or arrayed microdroplets in biosensors, biophysics, biology, and tissue engineering. This review is an extended version of the article \"FLUID3EAMS: Fluid-Fluid Interfacial Energy Driven 3D Structure Emergence in a Micropillar Scaffold and Development in Bioengineering\" published in Seibutsu Butsuri (vol. 62, p. 110-113, 2022).</p>","PeriodicalId":8976,"journal":{"name":"Biophysics and Physicobiology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2023-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10941964/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biophysics and Physicobiology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2142/biophysico.bppb-v20.0029","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/1/1 0:00:00","PubModel":"eCollection","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Herein, I review our recent work toward developing methods for generating three-dimensional (3D) droplet arrays driven by capillarity. Microdroplet array-based systems are useful for bioassays and bioengineering because they require only small amounts of samples and reagents and provide the high throughput. Various methods have been developed for preparing droplet arrays, among which methods based on capillarity have attracted considerable attention owing to their simplicity. I and collaborators have developed such methods based on capillary flow, including a method for preparing droplet arrays via oil-water replacement. We recently proposed our own concept of "fluid-fluid interfacial energy driven 3D structure emergence in a micropillar scaffold (FLUID3EAMS)" and its application. FLUID3EAMS allows a 3D droplet (or hydrogel bead) array to be generated in a micropillar scaffold by passing a fluid-fluid interface through the scaffold. This approach is useful for applications requiring ordered or arrayed microdroplets in biosensors, biophysics, biology, and tissue engineering. This review is an extended version of the article "FLUID3EAMS: Fluid-Fluid Interfacial Energy Driven 3D Structure Emergence in a Micropillar Scaffold and Development in Bioengineering" published in Seibutsu Butsuri (vol. 62, p. 110-113, 2022).
在此,我将回顾我们最近在开发由毛细管驱动的三维液滴阵列方法方面所做的工作。基于微液滴阵列的系统对生物测定和生物工程非常有用,因为它们只需要少量样品和试剂,而且通量高。目前已开发出多种制备液滴阵列的方法,其中基于毛细管的方法因其简便性而备受关注。我和合作者已经开发了基于毛细管流动的此类方法,包括通过油水置换制备液滴阵列的方法。最近,我们提出了自己的 "微柱支架中流体-流体界面能驱动的三维结构出现(FLUID3EAMS)"概念及其应用。FLUID3EAMS 允许通过流体-流体界面穿过微柱支架,从而在微柱支架中生成三维液滴(或水凝胶珠)阵列。这种方法适用于生物传感器、生物物理学、生物学和组织工程学中需要有序或阵列微滴的应用。本综述是文章 "FLUID3EAMS:Seibutsu Butsuri》(第 62 卷,第 110-113 页,2022 年)上发表的 "Fluid-Fluid Interfacial Energy Driven 3D Structure Emergence in a Micropillar Scaffold and Development in Bioengineering "一文的扩展版。