Conserved nucleotide sequence of NS5A in hepatitis G virus or GB virus C in Japan and China

International Hepatology Communications Pub Date : 1997-01-01 DOI:10.1016/S0928-4346(96)00338-6

Toshikazu Hada , Weidong Liu , Hiroyasu Imanishi , Kohya Fukui , Xiaohui Zhou , Zhancheng Yao , Qianhao Wu , Jiewen Qiu , Masao Ohno , Arata Iwasaki , Yoshiki Amuro , Yinrong Wu , Kazuya Higashino

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引用次数: 3

Abstract

A semi-nested PCR method was designed for detecting the NS5A region of hepatitis G virus (HGV) or GB virus C (GBV-C) using specific oligonucleotide primers. Patients, 14 of 69, suffering from hepatic diseases in Japan and 5 of 21 in China showed strong positive signals for HGV or GBV-C by this method. The PCR products were directly sequenced. The nucleotide sequences identified differed from one another by 0–5%, from HGV by 3–9% and GBV-C by 2–7%, the percentages of the latter two being compared with the sequences reported previously. Amino acid sequences were the same or had only one alteration compared with HGV and two to three alterations compared with GBV-C. These results indicate that the nucleotide sequence of NS5A of HGV (or GBV-C) is more conserved than that of NS3 of GBV-C (or HGV) which has been reported to show variations of 0–23%.

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日本和中国G型肝炎病毒和GB型肝炎病毒NS5A的保守核苷酸序列

设计了一种利用特异性寡核苷酸引物检测G型肝炎病毒(HGV)或GB病毒C (GBV-C) NS5A区的半巢式PCR方法。日本69名肝病患者中有14名，中国21名肝病患者中有5名，通过该方法显示出强烈的HGV或GBV-C阳性信号。PCR产物直接测序。所鉴定的核苷酸序列之间的差异为0-5%，与HGV的差异为3-9%，与GBV-C的差异为2-7%，后两者的百分比与之前报道的序列进行比较。与HGV相比，氨基酸序列相同或只有一个改变，与GBV-C相比有2到3个改变。这些结果表明，HGV(或GBV-C)的NS5A核苷酸序列比GBV-C(或HGV)的NS3核苷酸序列更为保守，已报道的变异幅度为0-23%。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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International Hepatology Communications

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