Rapid Screening Tolerance of 19 Soybean Varieties to Drought in the Germination Phase

H. Pujiwati, A. Romeida, Widodo, W. Prameswari, M. Husna, Anandyawati
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Abstract

Drought stress is an inhibiting factor for plant growth and development. Drought stress reduces the seed to germinate and to grow on different varieties. Response to germination to drought stress is the beginning signal of drought tolerance for several varieties of plants. The purposes of this study were to evaluate the germination response of 16 soybean varieties, to determine the degree of drought stress tolerance of 19 soybean varieties, and to identify varieties that are tolerant as well as sensitive to drought which stimulated by polyethylene glycol (PEG). Soybean seeds were added to sand media containing PEG (6000) at concentrations of 0%, 5%, 10%, 15%, and 20% which were equivalent to 0, -0.03 Mpa, -0.19 Mpa, -041 and 067 Mpa for 8 days. The germination responses by calculating the vigor index, germination, root length, hypocotyl length, root length and hypocotyl length ratio, fresh weight sprouts. Inhibition of germination increases with a reduction in water potential. Each soybean seed tested showed different germination responses which were different under drought stress conditions. The results of 19 varieties showed only one variety which was identified to be drought-tolerant, however, 9 varieties were identified as medium droughttolerant, and 9 varieties were identified as sensitive to drought. The variety that was tolerant to drought was Gepak Kuning. Varieties that were sensitive to drought were Dena 2, Dega 1, Wilis, Edamame, Devatra 1, Devatra 2, Grobogan, Anjasmoro, and Detam 1.
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19个大豆品种萌发期抗旱能力的快速筛选
干旱胁迫是植物生长发育的抑制因子。干旱胁迫减少了种子的发芽和在不同品种上的生长。发芽对干旱胁迫的反应是一些植物品种抗旱的开始信号。对16个大豆品种的萌发响应进行了评价,确定了19个大豆品种对干旱胁迫的耐受程度,并鉴定了对聚乙二醇(PEG)刺激下干旱的耐受性和敏感性。将大豆种子分别以0、-0.03 Mpa、-0.19 Mpa、-041和067 Mpa的浓度分别为0%、5%、10%、15%和20%的PEG(6000)添加到含PEG(6000)的砂介质中8 d。通过计算活力指数、发芽率、根长、下胚轴长、根长与下胚轴长比值、芽鲜重等指标对发芽反应进行研究。随着水势的降低,萌发抑制增加。不同的大豆种子在不同的干旱胁迫条件下表现出不同的发芽反应。19个品种的结果显示,只有1个品种被鉴定为耐旱品种,9个品种被鉴定为中等耐旱品种,9个品种被鉴定为干旱敏感品种。耐旱品种为格柏库宁。对干旱敏感的品种有Dena 2、Dega 1、Wilis、Edamame、Devatra 1、Devatra 2、Grobogan、Anjasmoro和Detam 1。
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