Deteção de vírus entéricos em amostras de água natural superficial e de água para consumo humano

Q3 Social Sciences Archivaria Pub Date : 2020-06-05 DOI:10.22181/aer.2020.0701
Daniel Salvador, M. Caeiro, J. Aguilar, Maria João Benoliel, Célia Neto
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Abstract

Water-related infectious diseases are important causes of morbidity and mortality. Enterovirus and Hepatitis A and E viruses are, among others, representative of the enteric viruses, which are disease-causing agents mainly transmitted by the oral-fecal route, through water. The objective of this study is to search for enteric viruses by reverse transcription followed by Real-time PCR (RT-qPCR), in samples of natural surface water and in drinking water. It is also intended to evaluate the eventual association of these viruses with other fecal contamination indicators, and the efficacy of the water treatment plants in their elimination. It was confirmed the adequacy of the methodology implemented since, in the 15 samples analyzed so far, Hepatitis A virus and Enterovirus RNAs were detected in natural surface water samples, in two and three samples, respectively. Both viruses RNAs were detected in one of these samples. No viral nucleic acids were detected in drinking water samples. Fecal coliforms (microbial indicators of fecal contamination) were detected in natural water samples, but not in drinking water samples. Viral RNA and coliform detection only partially co-occurred.
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天然地表水和饮用水样本中肠道病毒的检测
与水有关的传染病是造成发病率和死亡率的重要原因。除其他外,肠道病毒、甲型肝炎和戊型肝炎病毒是肠道病毒的代表,它们是主要通过水通过口腔-粪便途径传播的致病性病原体。本研究的目的是利用逆转录和实时荧光定量PCR (RT-qPCR)技术在天然地表水和饮用水样品中寻找肠道病毒。它还旨在评估这些病毒与其他粪便污染指标的最终关联,以及水处理厂消除它们的功效。在迄今分析的15个样本中,分别在两个和三个天然地表水样本中检测到甲型肝炎病毒和肠病毒rna,证实了所采用方法的适当性。在其中一个样本中检测到两种病毒rna。饮用水样品未检出病毒核酸。在天然水样中检测到粪便大肠菌群(粪便污染的微生物指标),而在饮用水样品中未检测到。病毒RNA和大肠菌群检测仅部分同时发生。
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来源期刊
Archivaria
Archivaria Social Sciences-Library and Information Sciences
CiteScore
0.80
自引率
0.00%
发文量
11
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