Pre Harvest Foliar Application of Methyl Jasmonate on Fruit Quality and Quality Enzymes and Phenolic Compounds Changes During Storage of Grapefruit

W. Ahmed
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Abstract

Grapefruit is an important fruit crop in the world as well as in Pakistan. The signalling molecule like methyl jasmonate (MeJA) are endogenous plant growth substances which can play a key role in plant growth and development and responses to environmental stresses. Phytochemicals in grapefruit are considered very important for human body due to their medicinal properties. The effects of methyl jasmonate (MeJA) applied before fifteen days of harvesting to estimate the fruit firmness, fruit color, total soluble solid (TSS), weight loss, total phenolic (TP) and separate phenolic compounds , total antioxidant activity (TAA) of “grape fruit were analyzed at initial stage of harvesting and after storage. Foliar spray of methyl jasmonate (MeJA) significantly decreased fruit weight loss after 90 days storage. Quality related parameters were maintained after 90 days storage. The higher doze of MeJA @ 4mM significantly maintained the total phenolics and antioxidant after 90 days of storage. Enzymes peroxidase (POD), catalase (CAT) and ascorbate peroxidase (APX), and superoxide dismutase (SOD), Polycultrase (Poly) were found higher at 90 days storage these enzymes play an important role to developed a defense mechanisms in stored fruit those were delay fruit repining and improve shelf life of grapefruit during storage. MeJA applications has significantly increased different individual phenolic compounds especially chlorogenic acid contents after 90 days storage. These phytonutrient have significant role for antioxdative stress anti-viral, anti-cancer, anti-inflammatory activities of human body. The application of MeJA has potential to produce the different enzymes linkage which increased the fruit internal change and produce the resistance for disease resistance and improve the quality of fruits.
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采前叶面施用茉莉酸甲酯对果实品质的影响及柚子贮藏期间品质酶和酚类物质的变化
葡萄柚是世界上重要的水果作物,在巴基斯坦也是如此。茉莉酸甲酯(MeJA)等信号分子是植物内源生长物质,在植物生长发育和对环境胁迫的响应中起关键作用。葡萄柚中的植物化学物质因其药用特性而被认为对人体非常重要。分析了采前15天施用茉莉酸甲酯(MeJA)对葡萄果实硬度、果实颜色、总可溶性固形物(TSS)、减重、总酚(TP)和分离酚类化合物、总抗氧化活性(TAA)的影响。叶面喷施茉莉酸甲酯(MeJA)可显著降低果实贮藏90 d后的失重。90天后,质量相关参数保持不变。高剂量的MeJA @ 4mM在90天后显著维持了总酚和抗氧化剂的含量。贮藏90 d后,过氧化物酶(POD)、过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)、超氧化物歧化酶(SOD)、多培养酶(Poly)等酶的含量显著升高,这些酶在贮藏果实中形成了延缓果实腐烂、延长贮藏果实保质期的防御机制。在贮藏90 d后,MeJA显著提高了不同酚类化合物的含量,尤其是绿原酸的含量。这些植物营养素对人体抗氧化应激、抗病毒、抗癌、抗炎等具有显著作用。MeJA的应用有可能产生不同的酶连锁,增加果实内部的变化,产生抗病性,提高果实品质。
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