The Effect of some nanoparticles on multiple antibiotics resistant Pseudomonas aeruginosa isolates

Abstract

One hundred seventy-nine clinical samples collected included: urine, wounds, pus, burns and tonsils from patients coming to Rizgary Teaching Hospital and Rozhawa Hospital in Erbil city from March to September 2013, (24) (13.40%) strain of Pseudomonas aeruginosa identified according to cultural characteristics, microscopically features and biochemical tests in addition to the API -20E.Antibiotics sensitivity done against 12 types of antibiotics. The results showed absolute resistance against Ampicillin (AM / 10μg)& Amoxicillin (AX / 25μg) by 100% whereas the lowest resistant appeared against Gentamicin(GM/10µg), Cephalothin(KF/30µg) & Ciprofloxacin(CIP/5µg) (33.3,25,20.8%) respectively .Ten isolates were selected according to their pattern of the highest resistance as these showing multi-drug resistances and tested to specify their minimum inhibitory concentration (MIC) for the antibiotics and some types of Nanoparticles include Silver in different sizes (20, 90)nm, Zinc oxide in different sizes (20,30, 50~150)nm and titanium dioxide in different sizes (10, 50, 100)nm. The results showed that the MIC for Ag 20,90nm was between (650 -2600) μg/ml and the MIC for ZnO20nm was between (81.25 -2600) μg/ml but the MIC for ZnO30, 50~150nm between (325-2600) μg/ml and the MIC for TiO210nm was between (81.25 -2600) μg/ml, MIC of TiO250nm between (325-2600) μg/ml but MIC of TiO2100nm between (162.5-2600) μg/ml .Synergism effect between the antibiotics and the Nanoparticles showed high activity in inhibition of Pseudomonas aeruginosa growth.