Bin Liu, Xiaorui Shang, Xuting Zhang, Wen Shao, Lulu Ren, Guojing Li, Mulan Zhu, Ruigang Wang
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引用次数: 0
Abstract
Caragana korshinskii is a deciduous shrub with large eco-economic value and strong tolerance to abiotic stresses. However, the shortage of reliable genetic transformation technology severely hinders its research on stress tolerance mechanisms and stress-resistant gene mining and application. In this study, the embryonic tip of the C. korshinskii seedling was used as the initiating explant to get regenerated plant through the direct organogenesis pathway, which significantly shortened the culture cycle and set the foundation for investigation of Agrobacterium-mediated genetic transformation. Our results suggest that the embryonic tip possesses robust meristem capacity and is an efficient method for transgenic breeding. This research provides a technical basis for asexual reproduction, molecular breeding, and gene function investigation in C. korshinskii by establishing, for the first time, an effective in vitro regeneration system and an Agrobacterium-mediated stable genetic transformation system utilizing the embryonic tip of C. korshinskii as explants.
期刊介绍：
Order presents the most original and innovative research on ordered structures and the use of order-theoretic methods in graph theory and combinatorics, lattice theory and algebra, set theory and relational structures, and the theory of computing. In each of these categories, we seek submissions that make significant use of orderings to study mathematical structures and processes. The interplay of order and combinatorics is of particular interest, as are the application of order-theoretic tools to algorithms in discrete mathematics and computing. Articles on both finite and infinite order theory are welcome.
The scope of Order is further defined by the collective interests and expertise of the editorial board, which are described on these pages. Submitting authors are asked to identify a board member, or members, whose interests best match the topic of their work, as this helps to ensure an efficient and authoritative review.
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