An Exosomal miRNA Biomarker for the Detection of Pancreatic Ductal Adenocarcinoma.

IF 1.7 4区农林科学 Q2 MATERIALS SCIENCE, PAPER & WOOD Journal of Wood Chemistry and Technology Pub Date : 2022-10-06 DOI:10.3390/bios12100831

Amy Makler, Ramaswamy Narayanan, Waseem Asghar

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引用次数: 5

Abstract

Pancreatic ductal adenocarcinoma (PDAC) remains a difficult tumor to diagnose and treat. To date, PDAC lacks routine screening with no markers available for early detection. Exosomes are 40-150 nm-sized extracellular vesicles that contain DNA, RNA, and proteins. These exosomes are released by all cell types into circulation and thus can be harvested from patient body fluids, thereby facilitating a non-invasive method for PDAC detection. A bioinformatics analysis was conducted utilizing publicly available miRNA pancreatic cancer expression and genome databases. Through this analysis, we identified 18 miRNA with strong potential for PDAC detection. From this analysis, 10 (MIR31, MIR93, MIR133A1, MIR210, MIR330, MIR339, MIR425, MIR429, MIR1208, and MIR3620) were chosen due to high copy number variation as well as their potential to differentiate patients with chronic pancreatitis, neoplasms, and PDAC. These 10 were examined for their mature miRNA expression patterns, giving rise to 18 mature miRs for further analysis. Exosomal RNA from cell culture media was analyzed via RTqPCR and seven mature miRs exhibited statistical significance (miR-31-5p, miR-31-3p, miR-210-3p, miR-339-5p, miR-425-5p, miR-425-3p, and miR-429). These identified biomarkers can potentially be used for early detection of PDAC.

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用于检测胰腺导管腺癌的外泌体 miRNA 生物标记物

胰腺导管腺癌（PDAC）仍然是一种难以诊断和治疗的肿瘤。迄今为止，PDAC 缺乏常规筛查，没有可用于早期检测的标记物。外泌体是 40-150 nm 大小的细胞外囊泡，含有 DNA、RNA 和蛋白质。这些外泌体由所有类型的细胞释放到血液循环中，因此可以从患者体液中获取，从而有助于采用非侵入性方法检测 PDAC。我们利用公开的 miRNA 胰腺癌表达和基因组数据库进行了生物信息学分析。通过这项分析，我们确定了 18 种具有检测 PDAC 强大潜力的 miRNA。在这项分析中，我们选择了 10 个 miRNA（MIR31、MIR93、MIR133A1、MIR210、MIR330、MIR339、MIR425、MIR429、MIR1208 和 MIR3620），因为它们具有高拷贝数变异以及区分慢性胰腺炎、肿瘤和 PDAC 患者的潜力。对这 10 个 miRNA 进行了成熟 miRNA 表达模式的研究，得出了 18 个成熟的 miRs 供进一步分析。通过 RTqPCR 分析了细胞培养基中的外泌体 RNA，其中 7 个成熟 miRs 具有统计学意义（miR-31-5p、miR-31-3p、miR-210-3p、miR-339-5p、miR-425-5p、miR-425-3p 和 miR-429）。这些已确定的生物标志物可用于早期检测 PDAC。

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来源期刊

Journal of Wood Chemistry and Technology 工程技术-材料科学：纸与木材

CiteScore

3.70

自引率

20.00%

发文量

审稿时长

3 months

期刊介绍： The Journal of Wood Chemistry and Technology (JWCT) is focused on the rapid publication of research advances in the chemistry of bio-based materials and products, including all aspects of wood-based polymers, chemicals, materials, and technology. JWCT provides an international forum for researchers and manufacturers working in wood-based biopolymers and chemicals, synthesis and characterization, as well as the chemistry of biomass conversion and utilization. JWCT primarily publishes original research papers and communications, and occasionally invited review articles and special issues. Special issues must summarize and analyze state-of-the-art developments within the field of biomass chemistry, or be in tribute to the career of a distinguished researcher. If you wish to suggest a special issue for the Journal, please email the Editor-in-Chief a detailed proposal that includes the topic, a list of potential contributors, and a time-line.