DETAILED GC-MS ANALYSES OF THE „MYRTUS COMMUNIS“ L., ESSENTIAL OIL AND THE ANTIFUNGAL ACTIVITY ASSESSMENT

S. Filipović, T. Vasić, N. Radulović, D. Jevremović, I. Stanojević
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Abstract

Myrtus communis L., Myrtaceae, known as true myrtle, is a widely distributed evergreen shrub native to Mediterranean regions. This medicinal plant, used worldwide, possess a broad spectrum of phytochemical, pharmacological and therapeutic effects, mostly due to a large number of up to now detected, or isolated, essential oil components, considered the main biologically active ones. Multifarious secondary metabolite content, dependent on a geographic region, season of harvest and the length of distillation, depicts the variability of the essential oil composition obtained in general from its leaves, branches, fruits and flowers. Herein, we present the results of meticulous analyses of the essential oil (2.2%, w/w) constituents obtained from M. communis leaves, collected during the summer period from the coastal regions of peninsula Luštica, Montenegro. Detailed GC-MS analyses enabled the identification of 66 constituents, among which 1,8-cineole (28.4%), linalool (18.4%), α-pinene (16.6%), geranyl acetate (6.6%), α-terpineol (6.3%) and linalyl acetate (4.2%) were the major ones. Together with the secondary metabolite profile, determination of the Montenegro true myrtle essential oil agricultural plant protection potential is also estimated, by assessing mycelia growth of Colletotrichum acutatum J.H. Simmonds C.A.2 isolates, causative of strawberry antrachnose, in vitro.
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桃金娘挥发油的gc-ms分析及抗真菌活性评价
Myrtus communis L.,桃金娘科,被称为真桃金娘,是一种广泛分布的常绿灌木,原产于地中海地区。这种药用植物在世界范围内使用,具有广泛的植物化学,药理和治疗作用,主要是由于大量迄今为止检测到或分离到的精油成分,被认为是主要的生物活性成分。不同的次生代谢物含量,取决于地理区域、收获季节和蒸馏时间,描绘了从其叶子、树枝、果实和花朵中获得的精油成分的变化。在此,我们展示了对夏季期间从黑山Luštica半岛沿海地区收集的M. communis叶子中获得的精油(2.2%,w/w)成分的细致分析结果。经GC-MS分析,共鉴定出66种主要成分,其中1,8-桉树油脑(28.4%)、芳樟醇(18.4%)、α-蒎烯(16.6%)、乙酸香叶醇(6.6%)、α-松油醇(6.3%)和乙酸芳樟醇(4.2%)为主要成分。通过对草莓炭疽病菌acutatum J.H. Simmonds C.A.2分离株的体外菌丝生长情况进行评价,并结合次生代谢物谱,对黑山真桃金桃精油的农业植物保护潜力进行了评价。
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