Effect of Submerging Solanum lycopersicum Roots in Salicylic Acid (SA) Solution for Different Durations on Nematode Infection and Expressions of SlPR5 Gene

Abstract

Salicylic acid (SA) stimulates the mechanism of the plant defence and involves in a role in plant pathogen interactions. Plant parasitic nematodes are important biotic stresses causing negative effect on plant growth and development. Treatment of plant roots with SA may increase the plant defence mechanisms against biotic stresses. However, the treated effect of SA on plant defence mechanisms against a root-knot nematode, Meloidogyne incognita, has not been fully understood in terms of plant pathogen interactions. Therefore, this study was aimed to determine the most effective SA exposure time on increasing the plant defence and decreasing the nematode parasitism in Solanum lycopersicum. In addition, effects of SA treatment on the expression Pathogenesis Related Gene 5 (PR5) was evaluated. For this aim, tomato seedlings were exposed within 1000µM SA concentration with distinctive time durations. The expression of PR5 gene was accomplished using RT-PCR at 1, 3, 7, 14, 21 days post infection (dpi) for each sample. Root galling index, nematode number and reproduction rate were evaluated. Results revealed that nematode reproduction rate was decreased at in longer durations after SA treatment on roots. The highest nematode reproduction rate was determined in nematode+water (non-SA treatment) application compare to SA treatments. The highest increased level of expression of SlPR5 gene was determined in early (1 dpi) SA treatment + nematode infection. To conclude, SA treatment may increase the plant defence mechanisms and PR5 gene may involve in nematode-plant parasitism.