Cheng-ai Wu, M. Tsujita, K. Okumura‐Noji, S. Usui, Hajime Kakuuchi, M. Okazaki, S. Yokoyama
{"title":"Cholesteryl Ester Transfer Protein Expressed in Lecithin Cholesterol Acyltransferase–Deficient Mice","authors":"Cheng-ai Wu, M. Tsujita, K. Okumura‐Noji, S. Usui, Hajime Kakuuchi, M. Okazaki, S. Yokoyama","doi":"10.1161/01.ATV.0000026297.50542.62","DOIUrl":null,"url":null,"abstract":"Objective—Regulation of plasma cholesteryl ester transfer protein (CETP) concentration was studied in lecithin-cholesterol acyltransferase (LCAT)-knockout mice. Methods and Results—LCAT-knockout mice were cross-bred with CETP transgenic mice. The offspring (n=63) were classified for LCAT genotype and plasma CETP levels (no CETP, low CETP, and high CETP). High density lipoprotein (HDL) decreased as LCAT decreased in each CETP-level group. In the lcat(+/+) and lcat(+/ −) mice, plasma CETP varied from 0 to 30 &mgr;g/mL, whereas it was <10 &mgr;g/mL in the lcat( −/ −) mice. HDL cholesterol and phospholipid decreased and HDL triglyceride and apolipoprotein B increased in CETP in the lcat(+/+) and lcat(+/ −) mice, whereas there was no difference in HDL between low and high CETP. An effect of CETP on HDL was not detected in the lcat( −/ −) mice because of the absence of mature HDL. Genomic DNA and mRNA of CETP were correlated and were similar in the lcat( −/ −) and lcat(+/+) mice. Plasma CETP was correlated with its genomic DNA and mRNA, but the slope of the increase was much lower in the lcat( −/ −) mice. Whereas plasma CETP mostly associates with HDL in the lcat(+/+) mouse, it is found free in the lcat( −/ −) mouse. Conclusions—Plasma CETP is posttranscriptionally downregulated in the lcat( −/ −) mice, presumably by its extremely low HDL.","PeriodicalId":8418,"journal":{"name":"Arteriosclerosis, Thrombosis, and Vascular Biology: Journal of the American Heart Association","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2002-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"5","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Arteriosclerosis, Thrombosis, and Vascular Biology: Journal of the American Heart Association","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1161/01.ATV.0000026297.50542.62","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 5
Abstract
Objective—Regulation of plasma cholesteryl ester transfer protein (CETP) concentration was studied in lecithin-cholesterol acyltransferase (LCAT)-knockout mice. Methods and Results—LCAT-knockout mice were cross-bred with CETP transgenic mice. The offspring (n=63) were classified for LCAT genotype and plasma CETP levels (no CETP, low CETP, and high CETP). High density lipoprotein (HDL) decreased as LCAT decreased in each CETP-level group. In the lcat(+/+) and lcat(+/ −) mice, plasma CETP varied from 0 to 30 &mgr;g/mL, whereas it was <10 &mgr;g/mL in the lcat( −/ −) mice. HDL cholesterol and phospholipid decreased and HDL triglyceride and apolipoprotein B increased in CETP in the lcat(+/+) and lcat(+/ −) mice, whereas there was no difference in HDL between low and high CETP. An effect of CETP on HDL was not detected in the lcat( −/ −) mice because of the absence of mature HDL. Genomic DNA and mRNA of CETP were correlated and were similar in the lcat( −/ −) and lcat(+/+) mice. Plasma CETP was correlated with its genomic DNA and mRNA, but the slope of the increase was much lower in the lcat( −/ −) mice. Whereas plasma CETP mostly associates with HDL in the lcat(+/+) mouse, it is found free in the lcat( −/ −) mouse. Conclusions—Plasma CETP is posttranscriptionally downregulated in the lcat( −/ −) mice, presumably by its extremely low HDL.