Cholesteryl Ester Transfer Protein Expressed in Lecithin Cholesterol Acyltransferase–Deficient Mice

Cheng-ai Wu, M. Tsujita, K. Okumura‐Noji, S. Usui, Hajime Kakuuchi, M. Okazaki, S. Yokoyama
{"title":"Cholesteryl Ester Transfer Protein Expressed in Lecithin Cholesterol Acyltransferase–Deficient Mice","authors":"Cheng-ai Wu, M. Tsujita, K. Okumura‐Noji, S. Usui, Hajime Kakuuchi, M. Okazaki, S. Yokoyama","doi":"10.1161/01.ATV.0000026297.50542.62","DOIUrl":null,"url":null,"abstract":"Objective—Regulation of plasma cholesteryl ester transfer protein (CETP) concentration was studied in lecithin-cholesterol acyltransferase (LCAT)-knockout mice. Methods and Results—LCAT-knockout mice were cross-bred with CETP transgenic mice. The offspring (n=63) were classified for LCAT genotype and plasma CETP levels (no CETP, low CETP, and high CETP). High density lipoprotein (HDL) decreased as LCAT decreased in each CETP-level group. In the lcat(+/+) and lcat(+/ −) mice, plasma CETP varied from 0 to 30 &mgr;g/mL, whereas it was <10 &mgr;g/mL in the lcat( −/ −) mice. HDL cholesterol and phospholipid decreased and HDL triglyceride and apolipoprotein B increased in CETP in the lcat(+/+) and lcat(+/ −) mice, whereas there was no difference in HDL between low and high CETP. An effect of CETP on HDL was not detected in the lcat( −/ −) mice because of the absence of mature HDL. Genomic DNA and mRNA of CETP were correlated and were similar in the lcat( −/ −) and lcat(+/+) mice. Plasma CETP was correlated with its genomic DNA and mRNA, but the slope of the increase was much lower in the lcat( −/ −) mice. Whereas plasma CETP mostly associates with HDL in the lcat(+/+) mouse, it is found free in the lcat( −/ −) mouse. Conclusions—Plasma CETP is posttranscriptionally downregulated in the lcat( −/ −) mice, presumably by its extremely low HDL.","PeriodicalId":8418,"journal":{"name":"Arteriosclerosis, Thrombosis, and Vascular Biology: Journal of the American Heart Association","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2002-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"5","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Arteriosclerosis, Thrombosis, and Vascular Biology: Journal of the American Heart Association","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1161/01.ATV.0000026297.50542.62","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 5

Abstract

Objective—Regulation of plasma cholesteryl ester transfer protein (CETP) concentration was studied in lecithin-cholesterol acyltransferase (LCAT)-knockout mice. Methods and Results—LCAT-knockout mice were cross-bred with CETP transgenic mice. The offspring (n=63) were classified for LCAT genotype and plasma CETP levels (no CETP, low CETP, and high CETP). High density lipoprotein (HDL) decreased as LCAT decreased in each CETP-level group. In the lcat(+/+) and lcat(+/ −) mice, plasma CETP varied from 0 to 30 &mgr;g/mL, whereas it was <10 &mgr;g/mL in the lcat( −/ −) mice. HDL cholesterol and phospholipid decreased and HDL triglyceride and apolipoprotein B increased in CETP in the lcat(+/+) and lcat(+/ −) mice, whereas there was no difference in HDL between low and high CETP. An effect of CETP on HDL was not detected in the lcat( −/ −) mice because of the absence of mature HDL. Genomic DNA and mRNA of CETP were correlated and were similar in the lcat( −/ −) and lcat(+/+) mice. Plasma CETP was correlated with its genomic DNA and mRNA, but the slope of the increase was much lower in the lcat( −/ −) mice. Whereas plasma CETP mostly associates with HDL in the lcat(+/+) mouse, it is found free in the lcat( −/ −) mouse. Conclusions—Plasma CETP is posttranscriptionally downregulated in the lcat( −/ −) mice, presumably by its extremely low HDL.
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
卵磷脂胆固醇酰基转移酶缺陷小鼠中胆固醇酯转移蛋白的表达
目的:研究卵磷脂-胆固醇酰基转移酶(LCAT)敲除小鼠血浆胆固醇酯转移蛋白(CETP)浓度的变化。方法与结果:lcat基因敲除小鼠与CETP转基因小鼠杂交。后代(n=63)根据LCAT基因型和血浆CETP水平(无CETP、低CETP和高CETP)进行分类。各cetp水平组高密度脂蛋白(HDL)随LCAT的降低而降低。lcat(+/+)和lcat(+/−)小鼠血浆CETP变化范围为0 ~ 30 μ g/mL,而lcat(−/−)小鼠血浆CETP变化范围<10 μ g/mL。lcat(+/+)和lcat(+/−)小鼠的CETP中HDL胆固醇和磷脂降低,HDL甘油三酯和载脂蛋白B升高,而低CETP和高CETP之间HDL无差异。由于缺乏成熟的HDL,在lcat(−/−)小鼠中未检测到CETP对HDL的影响。在lcat(−/−)和lcat(+/+)小鼠中,基因组DNA和CETP mRNA呈相关且相似。血浆CETP与其基因组DNA和mRNA相关,但在lcat(−/−)小鼠中增加的斜率要低得多。血浆CETP在lcat(+/+)小鼠中主要与HDL相关,而在lcat(- /−)小鼠中则是游离的。结论:lcat(−/−)小鼠血浆CETP转录后下调,可能是由于其极低的HDL。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
Resistance to Neointimal Hyperplasia and Fatty Streak Formation in Mice With Adrenomedullin Overexpression Homocysteine Binds to Human Plasma Fibronectin and Inhibits Its Interaction With Fibrin Inflammation in Atherosclerosis: Lesion Formation in LDL Receptor–Deficient Mice With Perforin and Lystbeige Mutations Higher Usual Dietary Intake of Phytoestrogens Is Associated With Lower Aortic Stiffness in Postmenopausal Women Application of Ex Vivo Flow Chamber System for Assessment of Stent Thrombosis
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1