{"title":"Role of β-Cryptoxanthin as an Antioxidant and Its Ability to Bind with Transferrin","authors":"Anushka Ghosh, U. Hazra, D. Dutta","doi":"10.17706/ijbbb.2019.9.4.258-264","DOIUrl":null,"url":null,"abstract":"The potential nutraceutical benefits of carotenoids, which are versatile bioactive compounds, have been of great interest recently for its applications as dietary supplements. Carotenoids are red-orange coloured pigments which absorb light in the wavelength region of 400-550 nm. They are produced by plants, bacteria, algae and fungi and are abundantly distributed in the nature. In this study, our focus is on beta-cryptoxanthin (β-CRX), a yellow colored pro-vitamin A xanthophyll which is extracted from a previously isolated bacterium Kocuria marina DAGII grown in Brain Heart Infusion and sub-cultured in low cost dairy waste like whey and incubated at 25°C and 150 rpm for 5 days. The extracted beta-cryptoxanthin showed good radical scavenging activity and played a role in inhibition of lipid oxidation. Transferrin is a glycoprotein which plays a significant role in the mobilisation of iron in the body. It has two receptors TfR1 and TfR2 amongst which TfR1 binds to the iron-loaded transferrin. In cases of secondary hemochromatosis, HFE protein competes with transferrin to bind to TfR1 which leads to iron built up which is detrimental to the human body. Beta-cryptoxanthin was found to bind to Transferrin with a binding energy of -8.2 kcal/mol.","PeriodicalId":13816,"journal":{"name":"International Journal of Bioscience, Biochemistry and Bioinformatics","volume":"13 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Bioscience, Biochemistry and Bioinformatics","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.17706/ijbbb.2019.9.4.258-264","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1
Abstract
The potential nutraceutical benefits of carotenoids, which are versatile bioactive compounds, have been of great interest recently for its applications as dietary supplements. Carotenoids are red-orange coloured pigments which absorb light in the wavelength region of 400-550 nm. They are produced by plants, bacteria, algae and fungi and are abundantly distributed in the nature. In this study, our focus is on beta-cryptoxanthin (β-CRX), a yellow colored pro-vitamin A xanthophyll which is extracted from a previously isolated bacterium Kocuria marina DAGII grown in Brain Heart Infusion and sub-cultured in low cost dairy waste like whey and incubated at 25°C and 150 rpm for 5 days. The extracted beta-cryptoxanthin showed good radical scavenging activity and played a role in inhibition of lipid oxidation. Transferrin is a glycoprotein which plays a significant role in the mobilisation of iron in the body. It has two receptors TfR1 and TfR2 amongst which TfR1 binds to the iron-loaded transferrin. In cases of secondary hemochromatosis, HFE protein competes with transferrin to bind to TfR1 which leads to iron built up which is detrimental to the human body. Beta-cryptoxanthin was found to bind to Transferrin with a binding energy of -8.2 kcal/mol.