{"title":"Cascade-Cas3 facilitates high-accuracy genome engineering in <i>Pseudomona</i>s using phage-encoded homologous recombination.","authors":"Wentao Zheng, Yandong Xia, Xue Wang, Shiqing Gao, Diao Zhou, Jun Fu, Ruijuan Li, Jia Yin","doi":"10.1016/j.engmic.2022.100046","DOIUrl":null,"url":null,"abstract":"<p><p>Phage-encoded homologous recombination (PEHR) is an efficient tool for bacterial genome editing. We previously developed and utilized a <i>Pseudomonas</i>-specific PEHR system. However, when using the PEHR system for <i>Pseudomonas</i> genome editing, false positives can be a problem. In this study, we combined a compact Cascade-Cas3 system from <i>P. aeruginosa</i> (PaeCas3c) with a <i>Pseudomonas</i>-specific PEHR system, and the results of our recombineering assay showed that this compact Cascade-Cas3 system can significantly improve PEHR recombineering accuracy.</p>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"10 1","pages":"100046"},"PeriodicalIF":0.0000,"publicationDate":"2022-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11611027/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Engineering Microbiology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1016/j.engmic.2022.100046","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2022/12/1 0:00:00","PubModel":"eCollection","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Phage-encoded homologous recombination (PEHR) is an efficient tool for bacterial genome editing. We previously developed and utilized a Pseudomonas-specific PEHR system. However, when using the PEHR system for Pseudomonas genome editing, false positives can be a problem. In this study, we combined a compact Cascade-Cas3 system from P. aeruginosa (PaeCas3c) with a Pseudomonas-specific PEHR system, and the results of our recombineering assay showed that this compact Cascade-Cas3 system can significantly improve PEHR recombineering accuracy.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
