ADDITIVES FOR CELLULASE ENHANCEMENT

Eugene M Obeng, Chan Yi Wei, Siti Nurul Nadzirah Adam, Clarence M. Ongkudon
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Abstract

Cellulases have been vital for the saccharification of lignocellulosic biomass into reduced sugars to produce biofuels and other essential biochemicals. However, the sugar yields achievable for canonical cellulases (i.e. endoglucanases, exoglucanases and β-glucosidases) have not been convincing in support of the highly acclaimed prospects and end-uses heralded. The persistent pursuit of the biochemical industry to obtain high quantities of useful chemicals from lignocellulosic biomass has resulted in the supplementation of cellulose-degrading enzymes with other biologicalcomplementation. Also, chemical additives (e.g. salts, surfactants and chelating agents) have been employed to enhance the stability and improve the binding and overall functionality of cellulases to increase product titre. Herein, we report the roadmap of cellulase-additive supplementations and the associated yield performances.
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纤维素酶增强添加剂
纤维素酶在将木质纤维素生物质糖化成还原糖以生产生物燃料和其他必需的生物化学物质方面起着至关重要的作用。然而,典型纤维素酶(即内切葡聚糖酶、外切葡聚糖酶和β-葡萄糖苷酶)可实现的糖产量并不能令人信服地支持高度赞扬的前景和所预示的最终用途。生物化学工业对从木质纤维素生物质中获得大量有用化学物质的不懈追求,导致了纤维素降解酶与其他生物补充物的补充。此外,化学添加剂(如盐,表面活性剂和螯合剂)已被用于提高稳定性,改善纤维素酶的结合和整体功能,以提高产品滴度。在此,我们报告了纤维素酶添加剂添加的路线图和相关的收率性能。
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