Raja Ratna Reddy Yakkanti, P. C. Sekhar, K. Reddy, S. Ramamoorthy, S. Suresh, T. Lakshmi, M. Rajesh, C. Reddy
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引用次数: 5
Abstract
D-Limonene is a dietary monoterpene with significant anticancer activity against many cancer types in preclinical and clinical studies. The study is designed to investigate synergistic anticancer effects of limonene and BEZ235 combination in COLO-320 and HCT-116 colon cancer cells. Cells were treated with both the drugs alone and in combination and the effects on cell viability; cell migration and clonogenic potential were examined. Results show that both drugs exhibited dose and time dependant cytotoxicity on the cell lines tested. CompuSyn analysis of the drug combination effects revealed the strong synergistic interaction of the combination. Our results also indicate that COLO-320 cells were more sensitive for anticancer effects of the drugs than HCT-116 cells. The presence of Ras and PI3K mutations in HCT-116 cells could possibly be one of the main reasons for the observed outcome as compared to the wild type expressions of them in COLO-320 cells.
d -柠檬烯是一种膳食单萜，在临床前和临床研究中对多种癌症具有显著的抗癌活性。本研究旨在探讨柠檬烯和BEZ235联合使用对COLO-320和HCT-116结肠癌细胞的协同抗癌作用。观察单药和联合用药对细胞活力的影响;检测细胞迁移和克隆潜能。结果表明，两种药物均表现出剂量和时间依赖性的细胞毒性。药物联合效应的CompuSyn分析显示该组合具有较强的协同作用。结果还表明，COLO-320细胞对药物的抗癌作用比HCT-116细胞更敏感。与COLO-320细胞中的野生型表达相比，HCT-116细胞中Ras和PI3K突变的存在可能是观察到结果的主要原因之一。
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